Hydrolysis characterization of phospholipid monolayers catalyzed by different phospholipases at the air-water interface
文献类型:期刊论文
作者 | He, Qiang; Li, Junbai |
刊名 | ADVANCES IN COLLOID AND INTERFACE SCIENCE
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出版日期 | 2007-02-28 |
卷号 | 131期号:1-2页码:91-98 |
关键词 | Enzymes Phospholipid Monolayer Interfacial Reaction Hydrolysis Molecular Recognition |
ISSN号 | 0001-8686 |
DOI | 10.1016/j.cis.2006.11.002 |
英文摘要 | Combination of some newly developed microscopic and spectroscopic techniques with conventional Langmuir monolayer method can provide more quantitative information with the molecular orientation and reorganization process of spread amphiphilic molecules at the air/water interface. These techniques are extended to investigate the hydrolysis process of spreading lipid monolayer catalyzed by different enzymes, phospholipases A(2), C and D, respectively. Synchrotron X-ray diffraction and infrared reflection absorption spectroscopy are able directly to give the structural information of the assembled monolayer, interfacial activity of amphiphiles and their components at the interface. Microscopic technique such as Brewster angle microscopy (BAM), fluorescence microscopy (FM) can be used to trace the morphological changes dynamically as the spreading lipid monolayer is hydrolyzed at the air/water interface. We summary here some latest progress in this filed and give a brief review over the hydrolysis features of phospholipid monolayer catalyzed by different enzymes. It is attempted to establish a model of membrane hydrolysis process in order to better understand the mechanism of membrane metabolism and signal transduction in a living system. (c) 2006 Elsevier B.V. All rights reserved. |
语种 | 英语 |
WOS记录号 | WOS:000245533100002 |
出版者 | ELSEVIER SCIENCE BV |
源URL | [http://ir.iccas.ac.cn/handle/121111/61975] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Li, Junbai |
作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Int Joint Lab CAS Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China 2.Max Planck Colloids & Interfaces, D-14476 Golm, Germany |
推荐引用方式 GB/T 7714 | He, Qiang,Li, Junbai. Hydrolysis characterization of phospholipid monolayers catalyzed by different phospholipases at the air-water interface[J]. ADVANCES IN COLLOID AND INTERFACE SCIENCE,2007,131(1-2):91-98. |
APA | He, Qiang,&Li, Junbai.(2007).Hydrolysis characterization of phospholipid monolayers catalyzed by different phospholipases at the air-water interface.ADVANCES IN COLLOID AND INTERFACE SCIENCE,131(1-2),91-98. |
MLA | He, Qiang,et al."Hydrolysis characterization of phospholipid monolayers catalyzed by different phospholipases at the air-water interface".ADVANCES IN COLLOID AND INTERFACE SCIENCE 131.1-2(2007):91-98. |
入库方式: OAI收割
来源:化学研究所
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