Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study
文献类型：期刊论文
| 作者 | Yang, Huayan; Yu, Junping; Fu, Guo; Shi, Xiaoli; Xiao, Lin; Chen, Yizhang; Fang, Xiaohong; He, Cheng |
| 刊名 | EXPERIMENTAL CELL RESEARCH
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 313期号:16页码:3497-3504 |
| 关键词 | Mac-1 Icam-1 Afm Single Molecule Dynamic Force Spectroscopy Binding Probability Rupture Force Activation Living Cell |
| ISSN号 | 0014-4827 |
| DOI | 10.1016/j.yexcr.2007.08.001 |
| 英文摘要 | The interaction between integrin macrophage differentiation antigen associated with complement three receptor function (Mac-1) and intercellular adhesion molecule-1 (ICAM-1), which is controlled tightly by the ligand-binding activity of Mac-1, is central to the regulation of neutrophil adhesion in host defense. Several "inside-out" signals and extracellular metal ions or antibodies have been found to activate Mac-1, resulting in an increased adhesiveness of Mac-1 to its ligands. However, the molecular basis for Mac-1 activation is not well understood yet. In this work, we have carried out a single-molecule study of Mac-1/ICAM-1 interaction force in living cells by atomic force microscopy (AFM). Our results showed that the binding probability and adhesion force of Mac-1 with ICAM-1 increased upon Mac-1 activation. Moreover, by comparing the dynamic force spectra of different Mac-1 mutants, we expected that Mac-1 activation is governed by the downward movement of its alpha 7 helix. (c) 2007 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000249797100011 |
| 出版者 | ELSEVIER INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62073]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Second Mil Med Univ, Inst Neurosci, Dept Neurobiol, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, State Key Lab High Field Laser Phys, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Huayan,Yu, Junping,Fu, Guo,et al. Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study[J]. EXPERIMENTAL CELL RESEARCH,2007,313(16):3497-3504. |
| APA | Yang, Huayan.,Yu, Junping.,Fu, Guo.,Shi, Xiaoli.,Xiao, Lin.,...&He, Cheng.(2007).Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study.EXPERIMENTAL CELL RESEARCH,313(16),3497-3504. |
| MLA | Yang, Huayan,et al."Interaction between single molecules of Mac-1 and ICAM-1 in living cells: An atomic force microscopy study".EXPERIMENTAL CELL RESEARCH 313.16(2007):3497-3504. |
入库方式： OAI收割
来源：化学研究所
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