Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells
文献类型：期刊论文
| 作者 | Yu, Junping; Wang, Qiang; Shi, Xiaoli; Ma, Xinyong; Yang, Huayan; Chen, Ye-Guang; Fang, Xiaohong |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2007-12-06 |
| 卷号 | 111期号:48页码:13619-13625 |
| ISSN号 | 1520-6106 |
| DOI | 10.1021/jp0758667 |
| 英文摘要 | Transforming growth factor beta 1 (TGF-beta 1) regulates many important cellular processes such as cell proliferation, differentiation, and apoptosis, etc. Its signaling is initiated by binding to and bringing together TGF-beta type II receptor (T beta RII) and type I receptor (T beta RI). However, it is not fully understood how the TGF-beta 1 ligand-receptor interaction occurs in living cells and what is the molecular mechanism of the signaling complex TGF-beta 1/T beta RII/T beta RI formation. In this study, we have investigated the interaction between TGF-beta 1 and its receptors in living cells with single-molecule force spectroscopy for the first time. By positioning TGF-beta 1-modified atomic force microscope (AFM) tips on the cells expressing fluorescent protein tagged TGF-beta receptors, the living-cell force measurement was realized with a combined fluorescence microscope and AFM. We found that coexpression of T beta RI with T beta RII enhanced the binding force of TGF-beta 1 with its receptors, hereas the expressed T beta RI itself exhibited no binding affinity to TGF-beta 1. Moreover, the unbinding dynamics of TGF-beta 1/T beta RII and TGF-beta 1/T beta RI/T beta RII were investigated with dynamic force spectroscopy under different AFM loading rates. The dissociation rate constants of TGF-beta 1 with its receptors as well as other parameters characterizing their dissociation pathways were obtained. The results suggested a more stable binding of TGF-beta 1 with the receptor after T beta RI is recruited and the important contribution of T beta RI to the signaling complex formation during TGF-beta I signaling. |
| 语种 | 英语 |
| WOS记录号 | WOS:000251291600020 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62195]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Tsing Hua Univ, Dept Biosci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Junping,Wang, Qiang,Shi, Xiaoli,et al. Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2007,111(48):13619-13625. |
| APA | Yu, Junping.,Wang, Qiang.,Shi, Xiaoli.,Ma, Xinyong.,Yang, Huayan.,...&Fang, Xiaohong.(2007).Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells.JOURNAL OF PHYSICAL CHEMISTRY B,111(48),13619-13625. |
| MLA | Yu, Junping,et al."Single-molecule force spectroscopy study of interaction between transforming growth factor beta 1 and its receptor in living cells".JOURNAL OF PHYSICAL CHEMISTRY B 111.48(2007):13619-13625. |
入库方式： OAI收割
来源：化学研究所
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