Selective modification of Trp19 in beta-lactoglobulin by a new diazo fluorescence probe
文献类型：期刊论文
| 作者 | Bao, Zhijuan; Wang, Shujuan; Shi, Wen; Dong, Suying; Ma, Huimin |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 6期号:9页码:3835-3841 |
| 关键词 | Analytical Methods Fluorescent Probe Beta-lactoglobulin Metallocarbene Selective Tryptophan Modification |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr070284n |
| 英文摘要 | To obtain the local information on the tryptophan domain in a protein, the design and synthesis of a new fluorescent probe, 1,7-bis(4-hydroxy-3-methoxyphenyl)-4-diazo-1,6-heptadiene-3,5-dione, is reported for the Selective modification of tryptophan residues. The probe comprises a curcumin fluorophore and a diazo labeling group, whose spectroscopic properties are characterized. The diazo group may be catalytically degraded by transition metal complexes such as Rh-2(OAc)(4), generating an active rhodium carbenoid intermediate, which can react selectively with tryptophan residues. By the use of the carbene's intermolecular reactions, the tryptophan residue (Trp19) of beta-lactoglobulin may be modified with the diazo curcumin probe. Furthermore, slight secondary but larger tertiary structural changes are detected after Trp19 is modified, and the Trp19 modification produces a great effect on the binding of 8-anilino-1-naphthalenesulfonic acid and retinol. These results indicate that the Trp19 residue plays an essential role in the structure and stability of beta-lactoglobulin, and the specific modification of this residue may have a potential use in further elucidating the relationship between the structure and function of the protein. |
| 语种 | 英语 |
| WOS记录号 | WOS:000249371000048 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62331]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Zhijuan,Wang, Shujuan,Shi, Wen,et al. Selective modification of Trp19 in beta-lactoglobulin by a new diazo fluorescence probe[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(9):3835-3841. |
| APA | Bao, Zhijuan,Wang, Shujuan,Shi, Wen,Dong, Suying,&Ma, Huimin.(2007).Selective modification of Trp19 in beta-lactoglobulin by a new diazo fluorescence probe.JOURNAL OF PROTEOME RESEARCH,6(9),3835-3841. |
| MLA | Bao, Zhijuan,et al."Selective modification of Trp19 in beta-lactoglobulin by a new diazo fluorescence probe".JOURNAL OF PROTEOME RESEARCH 6.9(2007):3835-3841. |
入库方式： OAI收割
来源：化学研究所
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