Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique
文献类型：期刊论文
| 作者 | Duan, Li; He, Qiang; Yan, Xuehai; Cui, Yue; Wang, Kewei; Li, Junbai |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007-03-09 |
| 卷号 | 354期号:2页码:357-362 |
| 关键词 | Microcapsules Hemoglobin Protein Glutaraldehyde Layer-by-layer Assembly |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.12.223 |
| 英文摘要 | Hemoglobin (Hb) protein microcapsules held together by cross-linker, glutaraldehyde (GA), were successfully fabricated by covalent layer-by-laver (LbL) technique. The Schiff base reaction occurred on the colloid templates between the aldehyde groups of GA and free amino sites of Hb results in the formation of GA/Hb microcapsules after the removal of the templates. The structure of obtained monodisperse protein microcapsule was characterized by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The UV-Vis spectra measurements demonstrate the existence of Hb in the assembled capsules. Cyclic voltammetry (CV) and potential-controlled amperometric measurements (I-t curve) confirm that hemoglobin microcapsules after fabrication remain their heme electroactivity. Moreover, direct electron transfer process from protein to electrode surface was performed to detect the heme electrochemistry without using any mediator or promoter. The experiments of fluorescence recovery after photobleaching (FRAP) by CLSM demonstrate that the hemoglobin protein microcapsules have an improved permeability comparing to the conventional polyelectrolyte microcapsules. (c) 2006 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000244050000006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62609]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Junbai |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid & Interface Sci, BNLMS,Int Joint Lab, Beijing 100080, Peoples R China 2.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany |
| 推荐引用方式 GB/T 7714 | Duan, Li,He, Qiang,Yan, Xuehai,et al. Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,354(2):357-362. |
| APA | Duan, Li,He, Qiang,Yan, Xuehai,Cui, Yue,Wang, Kewei,&Li, Junbai.(2007).Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,354(2),357-362. |
| MLA | Duan, Li,et al."Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 354.2(2007):357-362. |
入库方式： OAI收割
来源：化学研究所
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