Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide)
文献类型：期刊论文
| 作者 | Shen, Hong; Hu, Xixue; Yang, Fei; Bel, Jianzhong; Wang, Shenguo |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 28期号:29页码:4219-4230 |
| 关键词 | Plga Surface Modification Oxygen Plasma Treatment Cationized Gelatin Anchorage Cell Affinity Tissue Engineering |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2007.06.004 |
| 英文摘要 | Surface characteristics greatly influence attachment and growth of cells on biomaterials. Although polylactone-type biodegradable polymers have been widely used as scaffold materials for tissue engineering, lack of cell recognition sites, poor hydrophilicity and low surface energy lead to a bad cell affinity of the polymers, which limit the usage of polymers as scaffolds in tissue engineering. In the present study, surface Of Poly (L-lactide-co-glycolide) (PLGA) was modified by a method of combining oxygen plasma treatment with anchorage of cationized gelatin. Modification effect of the method was compared with other methods of oxygen plasma treatment, cationized gelatin or gelatin coating and combining oxygen plasma treatment with anchorage of gelatin. The change of surface property was compared by contact angles, surface energy, X-ray photoelectron spectra (XPS), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and scanning electron microscopy (SEM) measurement. The optimum oxygen pretreatment time determined by surface energy was 10 min when the power was 50 W and the oxygen pressure was 20 Pa. Analysis of the stability of gelatin and cationized gelatin anchored on PLGA by XPS, ATR-FTIR, contact angles and surface energy measurement indicated the cationized gelatin was more stable than gelatin. The result using mouse NIH 3T3 fibroblasts as model cells to evaluate cell affinity in vitro showed the cationized gelatin-anchored PLGA (OCG-PLGA) was more favorable for cell attachment and growth than oxygen plasma treated PLGA (O-PLGA) and gelatin-anchored PLGA (OG-PLGA). Moreover cell affinity of OCG-PLGA could match that of collagen-anchored PLGA (AC-PLGA). So the surface modification method combining oxygen plasma treatment with anchorage of cationized gelatin provides a universally effective way to enhance cell affinity of polylactone-type biodegradable polymers. (c) 2007 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000249605400006 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62767]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shenguo |
| 作者单位 | Chinese Acad Sci, Inst Chem, BNLMS, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Hong,Hu, Xixue,Yang, Fei,et al. Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide)[J]. BIOMATERIALS,2007,28(29):4219-4230. |
| APA | Shen, Hong,Hu, Xixue,Yang, Fei,Bel, Jianzhong,&Wang, Shenguo.(2007).Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide).BIOMATERIALS,28(29),4219-4230. |
| MLA | Shen, Hong,et al."Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide)".BIOMATERIALS 28.29(2007):4219-4230. |
入库方式： OAI收割
来源：化学研究所
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