Spectroscopic investigation on the interaction of J-aggregate with human serum albumin
文献类型：期刊论文
| 作者 | Zhang, Yazhou; Du, Hongyan; Tang, Yalin; Xu, Guangzhi; Yan, Wenpeng |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 128期号:2-3页码:197-203 |
| ISSN号 | 0301-4622 |
| 关键词 | Human Serum Albumin J-aggregation Interaction Induced Cd Apparent Constant |
| DOI | 10.1016/j.bpc.2007.04.002 |
| 英文摘要 | The interactions of three cyanine dyes, which exhibit different meso substituent in polymethine chain, with human serum albumin (HSA) have been investigated by the means of absorption, fluorescence and circular dichroism (CD) spectra. In phosphate buffer solution (PBS), the mentioned dyes exist not as isolated monomers but rather in the formation of J-aggregation. In the presence of HSA, the absorption and fluorescence emission spectra indicated that the J-aggregation was decomposed to monomer because of the strong affinity between dye molecules and HSA. Besides the association of cyanine dyes with HSA, binding to HSA gave rise to the J-aggregation CD signals. The meso substituent in the polymethine plays an important role in the interaction of HSA and the J-aggregation. Spectral studies showed that the dye bound with HSA in a 1:1 formation. The apparent constant (K.) value was roughly identified by analysis of the corresponding fluorescence data at various HSA concentrations. The higher affinity of the molecule with meso phenyl towards HSA with respect to molecules with meso ethyl or methyl can be attributed to the arrangement of molecules in J-aggregation and the hydrophobic force between the molecules and HSA. (c) 2007 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000247582800011 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62769]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Tang, Yalin |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, BNLMS, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yazhou,Du, Hongyan,Tang, Yalin,et al. Spectroscopic investigation on the interaction of J-aggregate with human serum albumin[J]. BIOPHYSICAL CHEMISTRY,2007,128(2-3):197-203. |
| APA | Zhang, Yazhou,Du, Hongyan,Tang, Yalin,Xu, Guangzhi,&Yan, Wenpeng.(2007).Spectroscopic investigation on the interaction of J-aggregate with human serum albumin.BIOPHYSICAL CHEMISTRY,128(2-3),197-203. |
| MLA | Zhang, Yazhou,et al."Spectroscopic investigation on the interaction of J-aggregate with human serum albumin".BIOPHYSICAL CHEMISTRY 128.2-3(2007):197-203. |
入库方式： OAI收割
来源：化学研究所
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