Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex
文献类型：期刊论文
| 作者 | Qi, Li; Han, Yanli; Zuo, Min; Chen, Yi |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 28期号:15页码:2629-2634 |
| 关键词 | Aromatic Amino Acid Enantioseparation Ligand-exchange Ce Zinc-l-lys Complex |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200600740 |
| 英文摘要 | A novel method of chiral ligand-exchange CE was developed with either L- or D-lysine (Lys) as a chiral ligand and zinc(II) as a central ion. This type of chiral complexes was explored for the first time to efficiently separate either individual pairs of or mixed aromatic amino acid enantiomers. Using a running buffer of 5 mM ammonium acetate, 100 mM boric acid, 3 mM ZnSO4 center dot 7H(2)O and 6 MM L-Lys at pH 7.6, unlabeled D,L-tryptophan, D,L-phenylalanine, and D,L-tyrosine were well separated, giving a chiral resolution of up to 7.09. The best separation was obtained at a Lys-to-zinc ratio of 2:1, zinc concentration of 2-4 mM and running buffer pH 7.6. The buffer pH was determined to have a strong influence on resolution, while buffer composition and concentration impacted on both the resolution and peak shape. Boric acid with some ammonium acetate was an adoptable buffer system, and some additives like ethylene diamine tetraacetic acid capable of destroying the complex should be avoided. Fine-tuning of the chiral resolution and elution order was achieved by regulating the ratio Of L-Lys to D-Lys; i.e. the resolution increased from zero to its highest value as the ratio ascended from 1:0 to 1:infinitive, and L-isomers eluted before or after D-isomers in excessive D- or L-Lys, respectively. |
| 语种 | 英语 |
| WOS记录号 | WOS:000248938400011 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62777]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Yi |
| 作者单位 | Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Han, Yanli,Zuo, Min,et al. Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex[J]. ELECTROPHORESIS,2007,28(15):2629-2634. |
| APA | Qi, Li,Han, Yanli,Zuo, Min,&Chen, Yi.(2007).Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex.ELECTROPHORESIS,28(15),2629-2634. |
| MLA | Qi, Li,et al."Chiral CE of aromatic amino acids by ligand-exchange with zinc(II)-L-lysine complex".ELECTROPHORESIS 28.15(2007):2629-2634. |
入库方式： OAI收割
来源：化学研究所
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