Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide
文献类型：期刊论文
| 作者 | Sun Li-Yan; Chu Tai-Wi; Wang Yi; Wang Xiang-Yun |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2007-04-10 |
| 卷号 | 28期号:4页码:621-626 |
| 关键词 | Peptide Phage Display Technology Peg I-131 Radioiodination Biodistribution |
| ISSN号 | 0251-0790 |
| 英文摘要 | A dodeca-peptide SVSVGMLPSHAP that targets human CL-187 tumor was identified in vivo by phage display technology. In order to evaluate the potency of YP13 as the imaging agent for diagnosis of tumor and to be easily radioiodinated, peptide YSVSVGMLPSHAP(YP13) with tyramine being added to the N-terminal was synthesized and chemically modified by conjugation with a low molecular weight monomethoxy polyethylene glycol( mPEG, M-w = 5000). YP13, mPEG-YP13 and YR13 ( a nonspecific random 13 peptide as a control peptide) were labeled with I-131 via Iodogen method. The labeled complex I-131-YP13, I-131-mPEG-YP13 and I-131-YR13 were isolated and purified by RP-HPLC. The radiochemical purity was estimated to be better than 95%. The RP-HPLC analysis of the supernatant of I-131-YP13 and I-131-mPEG-YP13 in serum collected for 60 min after injection shows that I-131-mPEG-YP13 in vivo was far more stable than I-131-YP13. The biodistribution of I-131-YP13, I-131-mPEG-YP13 and the control peptide I-131-YR13 in mice bearing human colon cancinoma was investigated. The experimental results indicate that there was higher uptake of I-131-YP13 and I-131-mPEG-YP13 in tumor than that of control peptide YR13 at 1 h and 2 h. The ratios of tumor to blood for I-131-mPEG-YP13 and I-131-YP13 increased as time prolonging. The longer serum half-life of mPEG-YP13 improved the uptake in tumor. Therefore, it can be expected that the radioiodinated YP13 and PEGylated YP13 may be a potential tumor-imaging agent. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245874300005 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62891]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Sun Li-Yan |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Dept Appl Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun Li-Yan,Chu Tai-Wi,Wang Yi,et al. Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(4):621-626. |
| APA | Sun Li-Yan,Chu Tai-Wi,Wang Yi,&Wang Xiang-Yun.(2007).Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(4),621-626. |
| MLA | Sun Li-Yan,et al."Radioiodination and biodistribution of PEG(y) lated YP13 polypeptide".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.4(2007):621-626. |
入库方式： OAI收割
来源：化学研究所
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