Binding of the bioactive component Aloe dihydroisocoumarin with human serum albumin
文献类型：期刊论文
| 作者 | Zhang, Xiu-Feng; Xie, Ling; Liu, Yang; Xiang, Jun-Feng; Tang, Ya-Lin |
| 刊名 | JOURNAL OF MOLECULAR STRUCTURE
 |
| 出版日期 | 2008-11-26 |
| 卷号 | 891期号:1-3页码:87-92 |
| 关键词 | Human Serum Albumin Aloe Dihydroisocoumarin Binding Protein Conformation Changes Docking |
| ISSN号 | 0022-2860 |
| DOI | 10.1016/j.molstruc.2008.03.005 |
| 英文摘要 | Aloe dihydroisocoumarin, one of new components isolated from Aloe vera, can scavenge reactive oxygen species. in order to explore the mechanism of drug action at a molecular level, the binding of Aloe dihydroisocoumarin with human serum albumin (HSA) has been investigated by using fluorescence ultraviolet (UV), circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy, fluorescence dynamics, and molecular dynamic docking for the first time. We observed a quenching of fluorescence of HSA in the presence of Aloe dihydroisocoumarin and also analyzed the quenching results using the Stern-Volmer equation and obtained high affinity binding to HSA. An isoemissive point at 414 nm is seen, indicating that the quenching of HSA fluorescence depends on the formation of Aloe dihydroisocoumarin HSA complex, which is further confirmed by fluorescence dynamic result. From the CD and FT-IR results, it is apparent that the interaction of Aloe dihydroisocoumarin with HSA causes a conformational change of the protein, with the gain of a-helix, It-sheet and random coil stability and the loss of p-turn content. Data obtained by fluorescence spectroscopy, fluorescence dynamics, CD, and FTIR experiments along with the docking studies Suggest that Aloe dihydroisocoumarin binds to residues located in subdomain IIA of HSA. (c) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000261537800013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/62997]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Tang, Ya-Lin |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiu-Feng,Xie, Ling,Liu, Yang,et al. Binding of the bioactive component Aloe dihydroisocoumarin with human serum albumin[J]. JOURNAL OF MOLECULAR STRUCTURE,2008,891(1-3):87-92. |
| APA | Zhang, Xiu-Feng,Xie, Ling,Liu, Yang,Xiang, Jun-Feng,&Tang, Ya-Lin.(2008).Binding of the bioactive component Aloe dihydroisocoumarin with human serum albumin.JOURNAL OF MOLECULAR STRUCTURE,891(1-3),87-92. |
| MLA | Zhang, Xiu-Feng,et al."Binding of the bioactive component Aloe dihydroisocoumarin with human serum albumin".JOURNAL OF MOLECULAR STRUCTURE 891.1-3(2008):87-92. |
入库方式： OAI收割
来源：化学研究所
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