Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore
文献类型：期刊论文
| 作者 | Leevy, W. Matthew1,2; Gammon, Seth T.3; Johnson, James R.1,2; Lampkins, Andrew J.1,2; Jiang, Hua1,2,4; Marquez, Manuel5,6; Piwnica-Worms, David3; Suckow, Mark A.7; Smith, Bradley D.1,2 |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 19期号:3页码:686-692 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/bc700376v |
| 英文摘要 | Optical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as light-emitting enzymes or fluorescent proteins. However, there are many circumstances where genetic reporters are not applicable, and there is a need for exogenous synthetic probes that can selectively target bacteria. The focus of this study is a fluorescent imaging probe that is composed of a bacterial affinity group conjugated to a near-infrared dye. The affinity group is a synthetic zinc (II) coordination complex that targets the anionic surfaces of bacterial cells. The probe allows detection of Staphylococcus aureus infection (5 x 10(7) cells) in a mouse leg infection model using whole animal near-infrared fluorescence imaging. Region of interest analysis showed that the signal ratio for infected leg to uninfected leg reaches 3.9 +/- 0.5 at 21 h postinjection of the probe. Ex vivo imaging of the organs produced a signal ratio of 8 for infected to uninfected leg. Immunohistochemical analysis confirmed that the probe targeted the bacterial cells in the infected tissue. Optimization of the imaging filter set lowered the background signal due to autofluorescence and substantially improved imaging contrast. The study shows that near-infrared molecular probes are amenable to noninvasive optical imaging of localized S. aureus infection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000254185000016 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63101]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Leevy, W. Matthew |
| 作者单位 | 1.Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA 2.Univ Notre Dame, Walther Canc Res Ctr, Notre Dame, IN 46556 USA 3.Washington Univ, Sch Med, Mallinckrodt Inst Radiol, Mol Imaging Ctr, St Louis, MO 63110 USA 4.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China 5.NIST, Ctr Theoret & Computat Nanosci, Gaithersburg, MD 20899 USA 6.Arizona State Univ, Harrington Dept Bioengn, Tempe, AZ 85287 USA 7.Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA |
| 推荐引用方式 GB/T 7714 | Leevy, W. Matthew,Gammon, Seth T.,Johnson, James R.,et al. Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore[J]. BIOCONJUGATE CHEMISTRY,2008,19(3):686-692. |
| APA | Leevy, W. Matthew.,Gammon, Seth T..,Johnson, James R..,Lampkins, Andrew J..,Jiang, Hua.,...&Smith, Bradley D..(2008).Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore.BIOCONJUGATE CHEMISTRY,19(3),686-692. |
| MLA | Leevy, W. Matthew,et al."Noninvasive optical Imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-zinc(II) affinity group conjugated to a near-infrared fluorophore".BIOCONJUGATE CHEMISTRY 19.3(2008):686-692. |
入库方式： OAI收割
来源：化学研究所
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