Electrochemical DNAzyme sensor for lead based on amplification of DNA-Au bio-bar codes
文献类型：期刊论文
| 作者 | Shen, Li1; Chen, Zhong1; Li, Yihan1; He, Shali1; Xie, Shubao1; Xu, Xiaodong1; Liang, Zhongwei1; Meng, Xin1; Li, Qing1; Zhu, Zhiwei1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008-08-15 |
| 卷号 | 80期号:16页码:6323-6328 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac800601y |
| 英文摘要 | An electrochemical DNAzyme sensor for sensitive and selective detection of lead ion (Pb2+) has been developed, taking advantage of catalytic reactions of a DNAzyme upon its binding to Ph2+ and the use of DNA-Au bio-bar codes to achieve signal enhancement. A specific DNAzyme for Ph2+ is immobilized onto an Au electrode surface via a thiol-Au interaction. The DNAzyme hybridizes to a specially designed complementary substrate strand that has an overhang, which in turn hybridizes to the DNA-Au bio-bar code (short oligonucleotides attached to 13 nm gold nanoparticles). A redox mediator, Ru(NH3)(6)(3+), which can bind to the anionic phosphate of DNA through electrostatic interactions, serves as the electrochemical signal transducer. Upon binding of Pb2+ to the DNAzyme, the DNAzyme catalyzes the hydrolytic cleavage of the substrate, resulting in the removal of the substrate strand along with the DNA bio-bar code and the bound Ru(NH3)(6)(3+) from the Au electrode surface. The release of Ru(NH3)(6)(3+) results in lower electrochemical signal of Ru(NH3)(6)(3+) confined on the electrode surface. Differential pulse voltammetry (DPV) signals of Ru(NH3)(6)(3+) provides quantitative measures of the concentrations of Pb2+, with a linear calibration ranging from 5 nM to 0.1 mu M. Because each nanoparticle carries a large number of DNA strands that bind to the signal transducer molecule Ru(NH3)(6)(3+), the use of DNA-Au bio-bar codes enhances the detection sensitivity by five times, enabling the detection of Pb2+ at a very low level (1 nM). The DPV signal response of the DNAzyme sensor is negligible for other divalent metal ions, indicating that the sensor is highly selective for Pb2+ Although this DNAzyme sensor is demonstrated for the detection of Pb2+, it has the potential to serve as a general platform for design sensors for other small molecules and heavy metal ions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000258448100024 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63357]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shao, Yuanhua |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2G3, Canada |
| 推荐引用方式 GB/T 7714 | Shen, Li,Chen, Zhong,Li, Yihan,et al. Electrochemical DNAzyme sensor for lead based on amplification of DNA-Au bio-bar codes[J]. ANALYTICAL CHEMISTRY,2008,80(16):6323-6328. |
| APA | Shen, Li.,Chen, Zhong.,Li, Yihan.,He, Shali.,Xie, Shubao.,...&Shao, Yuanhua.(2008).Electrochemical DNAzyme sensor for lead based on amplification of DNA-Au bio-bar codes.ANALYTICAL CHEMISTRY,80(16),6323-6328. |
| MLA | Shen, Li,et al."Electrochemical DNAzyme sensor for lead based on amplification of DNA-Au bio-bar codes".ANALYTICAL CHEMISTRY 80.16(2008):6323-6328. |
入库方式： OAI收割
来源：化学研究所
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