Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis
文献类型：期刊论文
| 作者 | Song, Liya1,2,3; Yuan, Hong-Jie1,4; Coffey, Lee1; Doran, John1; Wang, Mei-Xiang4; Qian, Shijun2; O'Reilly, Catherine1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 30期号:4页码:755-762 |
| ISSN号 | 0141-5492 |
| 关键词 | Activator Protein Enantioselectivity Groes-groel Nitrile Hydratase Rhodococcus Erythropolis |
| DOI | 10.1007/s10529-007-9611-3 |
| 英文摘要 | The genes encoding an enantioselective nitrile hydratase (NHase) from Rhodococcus erythropolis AJ270 have been cloned and an active NHase has been produced in Escherichia coli. Maximal activity was found when the genes encoding the alpha- and beta-subunits were transcribed as one unit and the gene encoding the P44k activator protein as a separate ORF on a single replicon. Addition of n-butyric acid and FeSO4 could improve NHase activity. Coexpression of the GroEL-GroES chaperone proteins increased activity in the absence of P44k protein but had no effect in the presence of P44k. The recombinant enzyme was highly enantioselective in the synthesis of S-(+)-3-benzoyloxy- 4-cyanobutyramide from the prochiral substrate 3-benzoyloxyglutaronitrile. |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000253357800028 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63375]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | O'Reilly, Catherine |
| 作者单位 | 1.Waterford Inst Technol, Dept Chem & Life Sci, Waterford, Ireland 2.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Liya,Yuan, Hong-Jie,Coffey, Lee,et al. Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis[J]. BIOTECHNOLOGY LETTERS,2008,30(4):755-762. |
| APA | Song, Liya.,Yuan, Hong-Jie.,Coffey, Lee.,Doran, John.,Wang, Mei-Xiang.,...&O'Reilly, Catherine.(2008).Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis.BIOTECHNOLOGY LETTERS,30(4),755-762. |
| MLA | Song, Liya,et al."Efficient expression in E-coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis".BIOTECHNOLOGY LETTERS 30.4(2008):755-762. |
入库方式： OAI收割
来源：化学研究所
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