Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation
文献类型：期刊论文
| 作者 | Feng, Fude1; Wang, Hongzhong2; Han, Lingli2; Wang, Shu1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2008-08-27 |
| 卷号 | 130期号:34页码:11338-11343 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/ja8011963 |
| 英文摘要 | A convenient, sensitive, and label-free method to determine the DNA methylation status of CpG sites of plasmid and human colon cancer cell has been developed. The system relies on highly selective single base extension reaction and significant optical amplification of cationic conjugated polyelectrolytes (CCP-1). The higher fluorescence resonance energy transfer efficiency between CCP-1 and fluorescein-labeled dGTP (dGTP-FI) is correlated to the incorporation of dGTP-FI into the probe DNA by single base extension reaction when the target/probe pair is complementary at the methylation site. As low as 1% methylation status can be determined by this new assay method. Because of the optical amplification property of CCP-1, the method exhibited high sensitivity with a concentration of analyte DNA at the picomolar level. The CCP-1 can form a complex with negatively charged DNA through electrostatic interactions, avoiding labeling the DNA target and probe by covalent linking. The isolation steps employed in other typical assays were avoided to simplify operations and increase repeatability. These features make the system promising for future use for early cancer diagnosis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000258660600033 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63523]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China 2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Fude,Wang, Hongzhong,Han, Lingli,et al. Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2008,130(34):11338-11343. |
| APA | Feng, Fude,Wang, Hongzhong,Han, Lingli,&Wang, Shu.(2008).Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,130(34),11338-11343. |
| MLA | Feng, Fude,et al."Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130.34(2008):11338-11343. |
入库方式： OAI收割
来源：化学研究所
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