Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples
文献类型：期刊论文
| 作者 | Li, Lin; Wang, Jianbin; Zhou, Shuang; Zhao, Meiping |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2008-07-14 |
| 卷号 | 620期号:1-2页码:1-7 |
| 关键词 | Immunoaffinity Monolith Bisphenol a Liquid Chromatography |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2008.05.036 |
| 英文摘要 | Matrix interference and contamination from analytical procedures are two major issues in the detection of trace level of bisphenol A (BPA) in environmental water. In this paper, we report a highly selective and efficient method for on-line extraction of BPA from water samples followed by quantification with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in 50 mm x 4.6 mm i.d. stainless steel cartridges and the epoxy-groups on the surface of the monolith were hydrolyzed and oxidized to aldehyde functions. Antibodies against BPA were covalently immobilized onto the monolithic column via Schiff base reaction. The optimum application buffer and elution buffer were found to be pH 5.5 phosphate buffered saline (PBS) and methanol-water (70:30, v/v), respectively. The obtained immunoaffinity monolithic columns were on-line coupled to LC-MS/MS using column-switching valves and the system was applied to analyze BPA in real environmental water samples. The method achieved a detection limit of 0.3 ng L-1 using a sample volume of 100 mL. The linear calibration range was 1.0-160 ng L-1. Samples including tap water, lake water and effluent from municipal sewage treatment plant were all measured with satisfactory results. (c) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000257553200001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63601]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, BNLMS, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lin,Wang, Jianbin,Zhou, Shuang,et al. Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples[J]. ANALYTICA CHIMICA ACTA,2008,620(1-2):1-7. |
| APA | Li, Lin,Wang, Jianbin,Zhou, Shuang,&Zhao, Meiping.(2008).Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples.ANALYTICA CHIMICA ACTA,620(1-2),1-7. |
| MLA | Li, Lin,et al."Development and characterization of an immunoaffinity monolith for selective on-line extraction of bisphenol A from environmental water samples".ANALYTICA CHIMICA ACTA 620.1-2(2008):1-7. |
入库方式： OAI收割
来源：化学研究所
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