Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II
文献类型：期刊论文
| 作者 | Bao, Han1; Zhang, Chunxi1; Kawakami, Keisuke2; Ren, Yanan1; Shen, Jian-Ren2; Zhao, Jingquan1 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 1777期号:9页码:1109-1115 |
| 关键词 | Photosystem Ii Tyrz Side-path Electron Donor Non-heme Iron Epr Electron Transfer |
| ISSN号 | 0005-2728 |
| DOI | 10.1016/j.bbabio.2008.04.044 |
| 英文摘要 | In intact PSII, both the secondary electron donor (Tyrz) and side-path electron donors (Car/Chl(Z)/Cyt(b559)) can be oxidized by P-680(+center dot) at cryogenic temperatures. In this paper, the effects of acceptor side, especially the redox state of the non-heme iron, on the donor side electron transfer induced by visible light at cryogenic temperatures were studied by EPR spectroscopy. We found that the formation and decay of the S(1)Tyr(Z). EPR signal were independent of the treatment of K3Fe(CN)(6), whereas formation and decay of the Car(+center dot)/Chl(Z)(+center dot) EPR signal correlated with the reduction and recovery of the Fe3+ EPR signal of the non-heme iron in K3Fe(CN)(6) pre-treated PSII, respectively. Based on the observed correlation between Car/Chl(Z) oxidation and Fe3+ reduction, the oxidation of non-heme iron by K3Fe(CN)(6) at 0 degrees C was quantified, which showed that around 50-60% fractions of the reaction centers gave rise to the Fe3+ EPR signal. In addition, we found that the presence of phenyl-p-benzoquinone significantly enhanced the yield of Tyr(Z) oxidation. These results indicate that the electron transfer at the donor side can be significantly modified by changes at the acceptor side, and indicate that two types of reaction centers are present in intact PSII, namely, one contains unoxidizable non-heme iron and another one contains oxidizable non-heme iron. Tyrz oxidation and side-path reaction occur separately in these two types of reaction centers, instead of competition with each other in the same reaction centers. In addition, our results show that the non-heme iron has different properties in active and inactive PSII. The oxidation of non-heme iron by K3Fe(CN)(6) takes place only in inactive PSII, which implies that the Fe3+ state is probably not the intermediate species for the turnover of quinone reduction. (C) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000259287200004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63607]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Chunxi |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Photochem, Beijing 100080, Peoples R China 2.Okayama Univ, Fac Sci, Grad Sch Nat Sci & Technol, Dept Biol, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Bao, Han,Zhang, Chunxi,Kawakami, Keisuke,et al. Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2008,1777(9):1109-1115. |
| APA | Bao, Han,Zhang, Chunxi,Kawakami, Keisuke,Ren, Yanan,Shen, Jian-Ren,&Zhao, Jingquan.(2008).Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1777(9),1109-1115. |
| MLA | Bao, Han,et al."Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1777.9(2008):1109-1115. |
入库方式： OAI收割
来源：化学研究所
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