Ultrafast isomerization dynamics of retinal in bacteriorhodopsin as revealed by femtosecond absorption spectroscopy
文献类型：期刊论文
| 作者 | Wu YiShi3; Zhong Sheng2; Ai XiCheng1; Hu KunSheng2; Zhang JianPing1 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 53期号:13页码:1972-1977 |
| 关键词 | Photoisomerization Photocycle Stimulated Emission Global Fitting |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-008-0283-8 |
| 英文摘要 | A femtosecond (fs) broad-band absorption apparatus was used to measure the early photoisomerization process of bacteriorhodopsin's (BR) photocycle to reveal the character of the important intermediate of J(625) and to obtain a deeper understanding of the role of photoisomerization in BR photocycle. Two time constants of 0.5 ps (95%) and 2.0 ps (5%) were brought out by global fitting on thirty curves in the near-infrared region. We suggest that the first time constant results from the decay of I(460) intermediate, and the longer component might be associated with BR isomer. The global analysis over 450, 540, 630, 710 and 870 nm traces identified two time constants, similar to 0.5 and similar to 3 ps. The slower component can be extracted from the processes of both J(625) -> BR(568) (540 nm) and J(625) -> K(590) (630 nm), suggesting J-intermediate takes a partial cis configuration. The obvious negative feature in early delay time of 700-780 nm regions was attributed to the radiative transition (stimulated emission) from the Franck-Condon active configuration along the isomerization potential surface of all-trans-retinal. |
| 语种 | 英语 |
| WOS记录号 | WOS:000257327400008 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63703]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ai XiCheng |
| 作者单位 | 1.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu YiShi,Zhong Sheng,Ai XiCheng,et al. Ultrafast isomerization dynamics of retinal in bacteriorhodopsin as revealed by femtosecond absorption spectroscopy[J]. CHINESE SCIENCE BULLETIN,2008,53(13):1972-1977. |
| APA | Wu YiShi,Zhong Sheng,Ai XiCheng,Hu KunSheng,&Zhang JianPing.(2008).Ultrafast isomerization dynamics of retinal in bacteriorhodopsin as revealed by femtosecond absorption spectroscopy.CHINESE SCIENCE BULLETIN,53(13),1972-1977. |
| MLA | Wu YiShi,et al."Ultrafast isomerization dynamics of retinal in bacteriorhodopsin as revealed by femtosecond absorption spectroscopy".CHINESE SCIENCE BULLETIN 53.13(2008):1972-1977. |
入库方式： OAI收割
来源：化学研究所
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