Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent
文献类型：期刊论文
| 作者 | Shu, Chun-Ying1; Ma, Xin-Yong1; Zhang, Jian-Fei2; Corwin, Frank D.3; Sim, Jae Hyun2; Zhang, Er-Yun1; Dorn, Harry C.2; Gibson, Harry W.2; Fatouros, Panos P.3; Wang, Chun-Ru1 |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 19期号:3页码:651-655 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/bc7002742 |
| 英文摘要 | Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)(16)(-)(NHCH2CH2COOH)(8), with the antibody of green fluorescence protein (anti-GFP), as a model for "tumor targeted" imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM(-1) s(-1)) than the parent gadofulleride aggregate (8.1 mM(-1) s(-1)) in phosphate buffered saline at 0.35 T, as also confirmed by T-1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent. |
| 语种 | 英语 |
| WOS记录号 | WOS:000254185000011 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63731]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shu, Chun-Ying |
| 作者单位 | 1.Chinese Acad Sci, BNLMS, Inst Chem, Beijing 100080, Peoples R China 2.Virginia Polytech Inst & State Univ, Dept Chem, Blacksburg, VA 24060 USA 3.Virginia Commonwealth Univ, Dept Radiol, Richmond, VA 23298 USA |
| 推荐引用方式 GB/T 7714 | Shu, Chun-Ying,Ma, Xin-Yong,Zhang, Jian-Fei,et al. Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent[J]. BIOCONJUGATE CHEMISTRY,2008,19(3):651-655. |
| APA | Shu, Chun-Ying.,Ma, Xin-Yong.,Zhang, Jian-Fei.,Corwin, Frank D..,Sim, Jae Hyun.,...&Fang, Xiao-Hong.(2008).Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent.BIOCONJUGATE CHEMISTRY,19(3),651-655. |
| MLA | Shu, Chun-Ying,et al."Conjugation of a water-soluble gadolinium endohedral fulleride with an antibody as a magnetic resonance imaging contrast agent".BIOCONJUGATE CHEMISTRY 19.3(2008):651-655. |
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来源：化学研究所
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