Cationic Conjugated Polyelectrolyte/Molecular Beacon Complex for Sensitive, Sequence-Specific, Real-Time DNA Detection
文献类型：期刊论文
| 作者 | Feng, Xuli; Duan, Xinrui; Liu, Libin; An, Lingling; Feng, Fude; Wang, Shu |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2008-11-04 |
| 卷号 | 24期号:21页码:12138-12141 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/la802932t |
| 英文摘要 | A new fluorescence method has been developed for DNA detection at room temperature in a sensitive, selective. economical, and real-time manner that interfaces the superiority of a molecular beacon in mismatch discrimination with the light-harvesting property of water-soluble conjugated polyelectrolytes. The probe solution contains a cationic conjugated polyelectrolyte (PFP-NMe3+), a molecular beacon with a five base pairs double-stranded stem labeled at the 5'-terminus with fluorescein (DNA(P-)Fl), and ethidium bromide (EB, a specific intercalator of dsDNA). The electrostatic interactions between DNA(P)-Fl and PFP-NMe3+ keep them in close proximity, facilitating the fluorescence resonance energy transfer (FRET) from PFP-NMe3+ to fluorescein. Upon adding a complementary strand to the probe Solution, the conformation of DNA(P)-Fl transits into dsDNA followed by the intercalation of EB into the grooves. Two-step FRET, from PFP-NMe3+ to DNA(P)-Fl (FRET-1), followed by FRET from DNA(P)-Fl to EB (FRET-2) takes place. In view of the observed fluorescein or EB emission changes, DNA can be detected in aqueous solution. Because the base mismatch in target DNA inhibits the transition of DNA(P)-Fl from the stem-loop to duplex structure, single nucleotide mismatch can be clearly detected. |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000260508800012 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63971]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ke Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Xuli,Duan, Xinrui,Liu, Libin,et al. Cationic Conjugated Polyelectrolyte/Molecular Beacon Complex for Sensitive, Sequence-Specific, Real-Time DNA Detection[J]. LANGMUIR,2008,24(21):12138-12141. |
| APA | Feng, Xuli,Duan, Xinrui,Liu, Libin,An, Lingling,Feng, Fude,&Wang, Shu.(2008).Cationic Conjugated Polyelectrolyte/Molecular Beacon Complex for Sensitive, Sequence-Specific, Real-Time DNA Detection.LANGMUIR,24(21),12138-12141. |
| MLA | Feng, Xuli,et al."Cationic Conjugated Polyelectrolyte/Molecular Beacon Complex for Sensitive, Sequence-Specific, Real-Time DNA Detection".LANGMUIR 24.21(2008):12138-12141. |
入库方式： OAI收割
来源：化学研究所
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