Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy
文献类型：期刊论文
| 作者 | Wang, Ying1; Li, Congjuan1; Li, Xiaohong1; Li, Yongfang3; Kraatz, Heinz-Bernhard2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008-03-15 |
| 卷号 | 80期号:6页码:2255-2260 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac7024688 |
| 英文摘要 | An unlabeled hairpin-DNA probe was used for the detection of eight single-nucleotide mismatches by electrochemical impedance spectroscopy (EIS). Upon hybridization of the target strand with the hairpin DNA probe, the stem-loop structure is opened and forms a duplex DNA. Accordingly, the film thickness is increased, which causes differences in the electrical properties of the film before and after hybridization. Randles equivalent circuits were employed to evaluate the EIS result. The differences in the charge-transfer resistance Delta R-CT between hairpin DNA (before hybridization) and duplex DNA (after hybridization) shows the consequence of a large structural rearrangement from hairpin to duplex. If a single-nucleotide mismatch is present in the center of the duplex, the difference in charge-transfer resistance Delta R-CT between B-DNA. in the absence and presence of Zn2+ allows the unequivocal detection of all eight single-nucleotide mismatches. The detection limit was measured, and Delta R-CT allows the discrimination of a single-nucleotide mismatch with the concentration of the target strand as low as 10 pM. |
| 语种 | 英语 |
| WOS记录号 | WOS:000254017000055 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/63983]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Xiaohong |
| 作者单位 | 1.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China 2.Univ Western Ontario, Dept Chem, London, ON N6A 5B7, Canada 3.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ying,Li, Congjuan,Li, Xiaohong,et al. Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy[J]. ANALYTICAL CHEMISTRY,2008,80(6):2255-2260. |
| APA | Wang, Ying,Li, Congjuan,Li, Xiaohong,Li, Yongfang,&Kraatz, Heinz-Bernhard.(2008).Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy.ANALYTICAL CHEMISTRY,80(6),2255-2260. |
| MLA | Wang, Ying,et al."Unlabeled hairpin-DNA probe for the detection of single-nucleotide mismatches by electrochemical impedance spectroscopy".ANALYTICAL CHEMISTRY 80.6(2008):2255-2260. |
入库方式： OAI收割
来源：化学研究所
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