Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation
文献类型：期刊论文
| 作者 | Chen, Zhiyong1; Xu, Li2; Liang, Yuan1; Wang, Jianbin1; Zhao, Meiping1; Li, Yuanzong1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2008-02-22 |
| 卷号 | 1182期号:1页码:128-131 |
| 关键词 | Hplc Monolith Cryogel Nanoparticle Protein |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2007.12.084 |
| 英文摘要 | A supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70 mm x 5.0 mm I.D.) using methacrylic acid (MAA) as functional monomer;and polyethylene glycol diacrylate (PEGDA) as crosslinker. The highly crosslinked (90%, molar ratio) poly(MAA-PEGDA) cryogel had more uniform supermacropores with a mean diameter of 25 p,m compared to the poly(acrylamide)-based cryogels. The viability of poly(MAA-PEGDA) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (CEW) and water-soluble poly(N-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (NIPAM-DMAPMA) nanoparticles from its crude reaction solution. The dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51 x 10(-3) mu mol/ml and 33.4 mu g/ml, respectively. The lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity. (c) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000253869800013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/64873]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China 2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhiyong,Xu, Li,Liang, Yuan,et al. Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1182(1):128-131. |
| APA | Chen, Zhiyong,Xu, Li,Liang, Yuan,Wang, Jianbin,Zhao, Meiping,&Li, Yuanzong.(2008).Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation.JOURNAL OF CHROMATOGRAPHY A,1182(1),128-131. |
| MLA | Chen, Zhiyong,et al."Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation".JOURNAL OF CHROMATOGRAPHY A 1182.1(2008):128-131. |
入库方式： OAI收割
来源：化学研究所
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