Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro
文献类型：期刊论文
| 作者 | Ni, Jin1; Wu, Qiuye2; Li, Yuguo3; Guo, Zhixin4; Tang, Gusheng1; Sun, Ding1; Gao, Fu1; Cai, Jianming1 |
| 刊名 | JOURNAL OF NANOPARTICLE RESEARCH
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 10期号:4页码:643-651 |
| 关键词 | Nano-c-60 Co-60 Gamma-irradiation Cytotoxicity Ros Radiosensitization Nanomedicine Health Implications |
| ISSN号 | 1388-0764 |
| DOI | 10.1007/s11051-007-9295-6 |
| 英文摘要 | There is growing evidence in recent years that the pristine fullerene may be endowed with strong pro-oxidant capacity to biological samples. In this investigation we tested the hypothesis that water-soluble fullerene-C-60 (nano-C-60) may interact with ionizing radiation enhancing its antiproliferative effects. The two tumor cell lines with different radiosensitivity B16 and SMMU-7721 were treated by a combination of pristine fullerene and Co-60 gamma irradiation. We measured cell survival rates, apoptotic characteristics, reactive oxygen species (ROS) production and alteration of cell diameter with or without gamma-irradiation. There was reduced survival with B16 and SMMU-7721 cells exposed to nano-C-60, with the inhibitory concentrations reducing the viability by 50% to 65 part per billion (ppb) and 150 ppb respectively. For cells exposed to nano-C-60 prior to gamma-irradiation, damage to cell membranes and increased numbers of apoptotic cells were detected by morphologic Hoechst-staining analysis and Annexin V/propidium iodide double-staining. In cells exposed to nano-C-60, there were increased levels of ROS, as measured by fluorescence detection under laser confocal microscopy. Preincubation with non-toxic pristine C-60 before gamma-ray caused enlargement of cells with increased diameter. The results show that nano-C-60 inhibits the growth of tumor cells at certain concentrations and increases the effects of Co-60 gamma-irradiation, possibly through the elevated production of cellular ROS and the membrane disruption. Data in this study indicates a possible consideration of using C-60 as a candidate of sensitization modifier in tumor radiation biology. |
| 语种 | 英语 |
| WOS记录号 | WOS:000253626900010 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65013]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cai, Jianming |
| 作者单位 | 1.Second Mil Med Univ, Dept Radiat Med, Shanghai 200433, Peoples R China 2.Second Mil Med Univ, Dept Organ Chem, Sch Pharm, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Lab Nanoscale Biomed, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 4.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, Jin,Wu, Qiuye,Li, Yuguo,et al. Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro[J]. JOURNAL OF NANOPARTICLE RESEARCH,2008,10(4):643-651. |
| APA | Ni, Jin.,Wu, Qiuye.,Li, Yuguo.,Guo, Zhixin.,Tang, Gusheng.,...&Cai, Jianming.(2008).Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro.JOURNAL OF NANOPARTICLE RESEARCH,10(4),643-651. |
| MLA | Ni, Jin,et al."Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro".JOURNAL OF NANOPARTICLE RESEARCH 10.4(2008):643-651. |
入库方式： OAI收割
来源：化学研究所
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