Cell-specific internalization study of an aptamer from whole cell selection
文献类型：期刊论文
| 作者 | Xiao, Zeyu1,2,3; Shangguan, Dihua1,2; Cao, Zehui1,2; Fang, Xiaohong3; Tan, Weihong1,2 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 14期号:6页码:1769-1775 |
| 关键词 | Cell Recognition Dna Recognition Dna Drug Delivery Internalization |
| ISSN号 | 0947-6539 |
| DOI | 10.1002/chem.200701330 |
| 英文摘要 | Nucleic acid aptamers have been shown many unique applications as excellent probes in molecular recognition. However, few examples are reported which show that aptamers can be internalized inside living cells for aptamer functional studies and for targeted intracellular delivery. This is mainly due to the limited number of aptamers available for cell-specific recognition, and the lack of research on their extra- and intracellular functions. One of the major difficulties in aptamers' in vivo application is that most of aptamers, unlike small molecules, cannot be directly taken up by cells without external assistance. In this work, we have studied a newly developed and cell-specific DNA aptamer, sgc8. This aptamer has been selected through a novel cell selection process (cell-SELEX), in which whole intact cells are used as targets while another related cell line is used as a negative control. The cell-SELEX enables generation of multiple aptamers for molecular recognition of the target cells and has significant advantages in discovering cell surface binding molecules for the selected aptamers. We have studied the cellular internalization of one of the selected aptamers. Our results show that sgc8 is internalized efficiently and specifically to the lymphoblastic leukemia cells. The internalized sgc8 aptamers are located inside the endosome. Comparison studies are done with the antibody for the binding protein of sgc8, PTK7 (Human protein tyrosine kinase-7) on cell surface. We also studied the internalization kinetics of both the aptamer and the antibody for the same protein on the living cell surface. We have further evaluated the effects of sgc8 on cell viability, and no cytotoxicity is observed. This study indicates that sgc8 is a promising agent for cell-type specific intracellular delivery. |
| 语种 | 英语 |
| WOS记录号 | WOS:000253470900009 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65273]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Tan, Weihong |
| 作者单位 | 1.Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Shands Canc Ctr, Gainesville, FL 32611 USA 2.Univ Florida, UF Genet Inst, McKnight Brain Inst, Gainesville, FL 32611 USA 3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Zeyu,Shangguan, Dihua,Cao, Zehui,et al. Cell-specific internalization study of an aptamer from whole cell selection[J]. CHEMISTRY-A EUROPEAN JOURNAL,2008,14(6):1769-1775. |
| APA | Xiao, Zeyu,Shangguan, Dihua,Cao, Zehui,Fang, Xiaohong,&Tan, Weihong.(2008).Cell-specific internalization study of an aptamer from whole cell selection.CHEMISTRY-A EUROPEAN JOURNAL,14(6),1769-1775. |
| MLA | Xiao, Zeyu,et al."Cell-specific internalization study of an aptamer from whole cell selection".CHEMISTRY-A EUROPEAN JOURNAL 14.6(2008):1769-1775. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。