Study of interaction between Smad7 and DNA by single-molecule force spectroscopy
文献类型：期刊论文
| 作者 | Shi, Xiaoli1; Chen, Feng2; Yu, Junping1; Xu, Yongchun1; Zhang, Suping2; Chen, Ye-Guang2; Fang, Xiaohong1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2008-12-26 |
| 卷号 | 377期号:4页码:1284-1287 |
| 关键词 | Tgf-beta Smad7 Smad4 Atomic Force Microscopy Single-molecule Force Spectroscopy Protein-dna Interaction |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2008.10.145 |
| 英文摘要 | Smad7 is an antagonist of TGF-beta signaling pathway and the mechanism of its inhibitory effect is of great interest. We recently found that Smad7 could function in the nucleus by binding to the DNA elements containing the minimal Smad binding element CAGA box. In this work, we further applied single-molecule force spectroscopy to study the DNA-binding property of Smad7. Smad7 showed similar binding strength to the oligonucleotides corresponding to the CAGA-containing activin responsive element (ARE) and the PAI-1 promoter, as that of Smad4. However, Smad7 also exhibited a binding activity to the mutant ARE with the CAGA sequence substituted, indicating its DNA-binding specificity is different from other Smads. Moreover, we demonstrated that the MH2 domain of Smad7 had a higher binding affinity to the DNA elements than the full-length Smad7, while the N-terminal domain exhibited an inhibitory effect. (C) 2008 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000261630900050 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65293]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiaoli,Chen, Feng,Yu, Junping,et al. Study of interaction between Smad7 and DNA by single-molecule force spectroscopy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,377(4):1284-1287. |
| APA | Shi, Xiaoli.,Chen, Feng.,Yu, Junping.,Xu, Yongchun.,Zhang, Suping.,...&Fang, Xiaohong.(2008).Study of interaction between Smad7 and DNA by single-molecule force spectroscopy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,377(4),1284-1287. |
| MLA | Shi, Xiaoli,et al."Study of interaction between Smad7 and DNA by single-molecule force spectroscopy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 377.4(2008):1284-1287. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。