Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis
文献类型：期刊论文
| 作者 | Deng, Wei; Cao, Aoneng; Lai, Luhua |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 17期号:6页码:1102-1105 |
| 关键词 | Amyloid Fibril Fret Classification Cross-beta Structure Elucidation |
| ISSN号 | 0961-8368 |
| DOI | 10.1110/ps.083475108 |
| 英文摘要 | The recently published microcrystal structures of amyloid fibrils from small peptides greatly enhanced our understanding of the atomic-level structure of the amyloid fibril. However, only a few amyloid fibrils can form microcrystals. The dansyl-tryptophan fluorescence resonance energy transfer (FRET) pair was shown to be able to detect the inter-peptide arrangement of the Transthyretin (105-115) amyloid fibril. In this study, we combined the known microcrystal structures with the corresponding FRET efficiencies to build a model for amyloid fibril structure classification. We found that fibrils with an antiparallel structural arrangement gave the largest FRET signal, those with a parallel arrangement gave the lowest FRET signal, and those with a mixed arrangement gave a moderate FRET signal. This confirms that the amyloid fibril structure patterns can be classified based on the FRET efficiency. |
| 语种 | 英语 |
| WOS记录号 | WOS:000256166600015 |
| 出版者 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65297]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lai, Luhua |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, State Ket Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Wei,Cao, Aoneng,Lai, Luhua. Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis[J]. PROTEIN SCIENCE,2008,17(6):1102-1105. |
| APA | Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2008).Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis.PROTEIN SCIENCE,17(6),1102-1105. |
| MLA | Deng, Wei,et al."Distinguishing the cross-beta spine arrangements in amyloid fibrils using FRET analysis".PROTEIN SCIENCE 17.6(2008):1102-1105. |
入库方式： OAI收割
来源：化学研究所
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