The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges
文献类型：期刊论文
| 作者 | Lu, Tiancheng1,2; Chen, Xuesi1; Shi, Quan1,2; Wang, Yu1; Zhang, Peibiao1; Jing, Xiabin1 |
| 刊名 | ACTA BIOMATERIALIA
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 4期号:6页码:1770-1777 |
| 关键词 | Electrospun Fibers Protein Immobilization Immunoassay Antibodies Antigens |
| ISSN号 | 1742-7061 |
| DOI | 10.1016/j.actbio.2008.05.006 |
| 英文摘要 | This paper aims at developing novel bioactive fibrous mats for protein immobilization and for protein separation/purification. For this purpose, an amphiphilic triblock copolymer, biotinylated poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) was co-electrospun together with poly(L-lactide-co-glycolide) into ultrafine fibers similar to 2 mu m in diameter, and a layer of blocking agent was coated on the fiber surfaces to block off possible non-specific binding of proteins. The biotin species retained their ability to specifically recognize and bind streptavidin, and the immobilized streptavidin could further combine with biotinylated antibodies, antigens and other biological moieties. Horseradish peroxidase-labeled streptavidin and fluorescein isothiocyanate-labeled goat globulin were used to detect the immobilizations of streptavidin and rabbit anti-goat IgG(H + L) via enzyme-linked immunoassay and confocal laser scanning microscope, respectively. The immobilized antigen was eluted from the fiber substrate with a glycine/HCl solution and the eluted antigen retained its bioactivity. Therefore, these biotin-carrying composite fibers have a variety of uses, including selective immobilization of functional proteins, antigen/antibody separation and purification, and vaccine preparation. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000261253400021 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65533]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jing, Xiabin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Tiancheng,Chen, Xuesi,Shi, Quan,et al. The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges[J]. ACTA BIOMATERIALIA,2008,4(6):1770-1777. |
| APA | Lu, Tiancheng,Chen, Xuesi,Shi, Quan,Wang, Yu,Zhang, Peibiao,&Jing, Xiabin.(2008).The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges.ACTA BIOMATERIALIA,4(6),1770-1777. |
| MLA | Lu, Tiancheng,et al."The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges".ACTA BIOMATERIALIA 4.6(2008):1770-1777. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。