On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids
文献类型：期刊论文
| 作者 | Qi, Li1,2,3; Yang, Gengliang1 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 30期号:16页码:2882-2889 |
| 关键词 | Dansyl Amino Acid Enantioseparation Ligand-exchange Ce On-column Labeling Zinc Complex |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200800753 |
| 英文摘要 | A novel on-column labeling method of amino acid (AA) enantiomers by using dansyl chloride (Dns-Cl) has been explored combined with chiral ligand-exchange CE (CLE-CE) technique and UV detection. Efficient labeling was achieved by sequential injection of buffer, Dns-Cl, AA enantiomers, Dns-Cl and buffer at 0.2 psi for 10.0, 3.0, 24.0, 3.0, and 10.0 s, respectively. After injection, the sandwich sections were allowed to react at room temperature for 35.0 min. With this procedure, successful on-column labeling and CLE-CE separation of 17 pairs AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO(4) and 6.0 MM L-Arg at pH 8.4, giving nine pairs fully enantioresolved with resolution in between 2.0 and 5.1. CLE-CE of some individual and mixed pairs was also demonstrated, much the same as using pre-column labeling. As validated by both artificially prepared solutions and serum samples, this new method was shown to be applicable to the quantitative analysis, with a linear range between 14.0 mu M and 3.7 mM, correlation coefficient above 0.99 and recovery in between 90.4% and 111.7%. It was also demonstrated that the migration time-temperature based curve allows for temperature determination in CE by using this new method. |
| 语种 | 英语 |
| WOS记录号 | WOS:000269712500015 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65799]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Gengliang |
| 作者单位 | 1.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China 3.Beijing Natl Lab Mol Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Yang, Gengliang. On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids[J]. ELECTROPHORESIS,2009,30(16):2882-2889. |
| APA | Qi, Li,&Yang, Gengliang.(2009).On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids.ELECTROPHORESIS,30(16),2882-2889. |
| MLA | Qi, Li,et al."On-column labeling technique and chiral ligand-exchange CE with zinc(II)-L-arginine complex as a chiral selector for assay of dansylated D,L-amino acids".ELECTROPHORESIS 30.16(2009):2882-2889. |
入库方式： OAI收割
来源：化学研究所
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