Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution
文献类型：期刊论文
| 作者 | Duan, Xinrui1; Yue, Wei2; Liu, Libing1; Li, Zhengping3; Li, Yuliang1; He, Fuchu2; Zhu, Daoben1; Zhou, Gangqiao2; Wang, Shu1 |
| 刊名 | NATURE PROTOCOLS
 |
| 出版日期 | 2009 |
| 卷号 | 4期号:6页码:984-991 |
| ISSN号 | 1754-2189 |
| DOI | 10.1038/nprot.2009.70 |
| 英文摘要 | This protocol describes a simple, convenient and sensitive single-nucleotide polymorphism (SNP) genotyping method using an optically amplifying cationic conjugated polymer and single base primer extension reaction. The fluorescence resonance energy transfer (FRET) efficiency between the conjugated polymer (PFP, poly{(1,4-phenylene)-2,7-[9,9-bis(6'-N, N, N-trimethyl ammonium)-hexyl fluorene] dibromide}) and a fluorescein-labeled dNTP (dNTP-Fl) is correlated to the incorporation of the dNTP-Fl into an allele-specific primer; incorporation occurs by a single base extension reaction when the target DNA and the primer are complementary at the SNP site. By triggering the FRET from PFP to fluorescein and measuring the change in fluorescence intensity of samples, the SNP genotypes can be discriminated. In comparison with other SNP genotyping methods, this protocol simplifies procedures and improves sensitivity by eliminating the need for primer labeling, cumbersome workups, chemical/enzymatic coupling reactions and sophisticated instruments. The assay takes about 2 h for PCR amplification followed by 5.5-7.5 h to obtain the genotypes. |
| 语种 | 英语 |
| WOS记录号 | WOS:000284097100002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65889]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China 2.Beijing Inst Radiat Med, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing, Peoples R China 3.Hebei Univ, Coll Chem & Environm Sci, Baoding, Hebei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Xinrui,Yue, Wei,Liu, Libing,et al. Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution[J]. NATURE PROTOCOLS,2009,4(6):984-991. |
| APA | Duan, Xinrui.,Yue, Wei.,Liu, Libing.,Li, Zhengping.,Li, Yuliang.,...&Wang, Shu.(2009).Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution.NATURE PROTOCOLS,4(6),984-991. |
| MLA | Duan, Xinrui,et al."Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution".NATURE PROTOCOLS 4.6(2009):984-991. |
入库方式： OAI收割
来源：化学研究所
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