中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Insight into the interaction of E. coli with gentamycin by ultrafast electrophoresis achieved within the microscopic visual field

文献类型:期刊论文

作者Xie, Mingyi1; Wang, Hai1; Chen, Yi1,2
刊名ELECTROPHORESIS
出版日期2009-11-01
卷号30期号:22页码:3884-3890
关键词IntE.action Of E. Coli With gE.tamycin Micro-video Detection Ultrafast Ce
ISSN号0173-0835
DOI10.1002/elps.200900183
英文摘要An ultrafast electrophoresis method was developed to have an insight into the interaction of E. coli, DH5 alpha, with an antibiotic gentamycin. The method used micro-video to visually measure the migration of bacteria, rather than molecules, within several micrometers, taking a time down to 0.04 s. Precise bacterial mobility was measured at the electric field strength of 42.5-62.5 V/cm, with a mobility deviation of less than 1.7% compared with a common CE method. The bacterial mobility measured this way was found to have a distribution increasing with the electric field strength applied, but the averaged mobility kept constant or independent of the electric field strength. As indicated by the bacterial mobility measured, DH5 alpha experienced four stages during interaction with the antibiotic gentamycin, namely, the outer surface adsorption equilibration, uptake or transferring of adsorbed gentamycin through cellular membrane, destruction of the membrane, and death of the bacteria. The bacterial outer surface adsorption of gentamycin was found to have duration up to about 5 min that decreased reversely with the concentration of gentamycin added. This proves that the activity of antibiotic gentamycin is dose-dependent.
语种英语
WOS记录号WOS:000272474900012
出版者WILEY-V C H VERLAG GMBH
源URL[http://ir.iccas.ac.cn/handle/121111/65933]  
专题中国科学院化学研究所
通讯作者Chen, Yi
作者单位1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100190, Peoples R China
2.Beijing Natl Lab Mol Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Xie, Mingyi,Wang, Hai,Chen, Yi. Insight into the interaction of E. coli with gentamycin by ultrafast electrophoresis achieved within the microscopic visual field[J]. ELECTROPHORESIS,2009,30(22):3884-3890.
APA Xie, Mingyi,Wang, Hai,&Chen, Yi.(2009).Insight into the interaction of E. coli with gentamycin by ultrafast electrophoresis achieved within the microscopic visual field.ELECTROPHORESIS,30(22),3884-3890.
MLA Xie, Mingyi,et al."Insight into the interaction of E. coli with gentamycin by ultrafast electrophoresis achieved within the microscopic visual field".ELECTROPHORESIS 30.22(2009):3884-3890.

入库方式: OAI收割

来源:化学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。