EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS
文献类型：期刊论文
| 作者 | Ke, Fu-you; Mo, Xiu-lei; Liang, De-hai1 |
| 刊名 | CHINESE JOURNAL OF POLYMER SCIENCE
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 27期号:5页码:601-610 |
| 关键词 | Persistence Length Overlap Concentration Polymer Matrix Dna Separation Electrophoresis |
| ISSN号 | 0256-7679 |
| DOI | 10.1142/S0256767909004291 |
| 英文摘要 | The persistence length and the overlapconcentration (c*) of poly(ethylene oxide) (PEO) and hydroxyethylcellulose (HEC) with similar molecular weight in 1 x TBE buffer were studied by laser light scattering and viscometry. Their effect on DNA separation was investigated by capillary electrophoresis. It was determined that the persistence length of HEC was at least 5 times higher than that of PEO. Therefore, the c* of HEC was smaller than that of PEO by a factor of ca. 2.5. It was also found that the c* values determined by laser light scattering were much lower than those by viscometry. We attributed it to the state of polymer chains in which c* was measured: laser light scattering determined the c* in ultradilute solution where the polymer chains were in the static state; while viscometry determined the c* in dilute or semidilute concentration region with polymer chains being in the hydrodynamic flowing state. When used in DNA separation, polymer chains were in different states due to the disturbance of DNA molecules, making c* region dependent. When the real concentration was normalized by c*, PEO and HEC showed almost equal performance in separating Phi X174/Hae III DNA digest in dilute solution, even though their persistence lengths were quite different. |
| 语种 | 英语 |
| WOS记录号 | WOS:000269451500001 |
| 出版者 | WORLD SCIENTIFIC PUBL CO PTE LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/65955]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liang, De-hai |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Dept Polymer Sci & Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Key Lab Polymer Chem & Phys, Minist Educ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Fu-you,Mo, Xiu-lei,Liang, De-hai. EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS[J]. CHINESE JOURNAL OF POLYMER SCIENCE,2009,27(5):601-610. |
| APA | Ke, Fu-you,Mo, Xiu-lei,&Liang, De-hai.(2009).EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS.CHINESE JOURNAL OF POLYMER SCIENCE,27(5),601-610. |
| MLA | Ke, Fu-you,et al."EFFECT OF OVERLAP CONCENTRATION AND PERSISTENCE LENGTH ON DNA SEPARATION IN POLYMER SOLUTIONS BY ELECTROPHORESIS".CHINESE JOURNAL OF POLYMER SCIENCE 27.5(2009):601-610. |
入库方式： OAI收割
来源：化学研究所
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