Chiral ligand-exchange CE assays for separation of amino acid enantiomers and determination of enzyme kinetic constant
文献类型：期刊论文
| 作者 | Qi, Li1,3; Qiao, Juan1,2; Yang, Gengliang3; Chen, Yi1 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 30期号:13页码:2266-2272 |
| 关键词 | D-amino Acid Oxidase Enantioseparation Enzyme Kinetics Ligand-exchange Ce Zinc Complex |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200800623 |
| 英文摘要 | This paper deals with studies on the use of Zn(II)-L-ornithine complex as a chiral selecting system for the enantioseparation and UV detection of amino acids (AAs) by using the principle of ligand-exchange CE. Successful enantioseparation of three pairs of label-free aromatic AAs and four pairs of labeled AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO(4) and 6.0 MM L-Orn at pH 8.2. This new method was shown to be applicable to the quantitative analysis Of D- and L-aromatic AAs, with a linear range between 12.5 and 800.0 mu g/mL, and a correlation coefficient above 0.99. Thus this assay, which is facile and relatively rapid, allows us to measure the enzyme catalytic activity in the incubation Of D,L-AAs with D-AA oxidase. Using this new method, we can determine the enzyme kinetic constant, lending insight into potential enzyme mechanism. |
| 语种 | 英语 |
| WOS记录号 | WOS:000268626900003 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66033]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Analyt Chem Life Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Hebei Univ, Coll Pharm, Baoding, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Qiao, Juan,Yang, Gengliang,et al. Chiral ligand-exchange CE assays for separation of amino acid enantiomers and determination of enzyme kinetic constant[J]. ELECTROPHORESIS,2009,30(13):2266-2272. |
| APA | Qi, Li,Qiao, Juan,Yang, Gengliang,&Chen, Yi.(2009).Chiral ligand-exchange CE assays for separation of amino acid enantiomers and determination of enzyme kinetic constant.ELECTROPHORESIS,30(13),2266-2272. |
| MLA | Qi, Li,et al."Chiral ligand-exchange CE assays for separation of amino acid enantiomers and determination of enzyme kinetic constant".ELECTROPHORESIS 30.13(2009):2266-2272. |
入库方式： OAI收割
来源：化学研究所
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