Effects of Quantum Dots in Polymerase Chain Reaction
文献类型：期刊论文
| 作者 | Wang, Libing1,2; Zhu, Yingyue1; Jiang, Yuan3; Qiao, Ruirui4; Zhu, Shuifang5; Chen, Wei1; Xu, Chuanlai1 |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2009-05-28 |
| 卷号 | 113期号:21页码:7637-7641 |
| ISSN号 | 1520-6106 |
| DOI | 10.1021/jp902404y |
| 英文摘要 | The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results showed that there was no enhancement. The mechanisms underlying, these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266296700031 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66057]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Wei |
| 作者单位 | 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China 2.Hunan Import & Export Inspect & Quarantine Bur, Changsha 410000, Hunan, Peoples R China 3.Jiangsu Import & Export Inspect & Quarantine Bur, Food Lab, Nanjing 210000, Jiangsu, Peoples R China 4.Chinese Acad Sci, Inst Chem, Beijing 100081, Peoples R China 5.Chinese Acad Inspect & Quarantine, Beijing 100082, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Libing,Zhu, Yingyue,Jiang, Yuan,et al. Effects of Quantum Dots in Polymerase Chain Reaction[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(21):7637-7641. |
| APA | Wang, Libing.,Zhu, Yingyue.,Jiang, Yuan.,Qiao, Ruirui.,Zhu, Shuifang.,...&Xu, Chuanlai.(2009).Effects of Quantum Dots in Polymerase Chain Reaction.JOURNAL OF PHYSICAL CHEMISTRY B,113(21),7637-7641. |
| MLA | Wang, Libing,et al."Effects of Quantum Dots in Polymerase Chain Reaction".JOURNAL OF PHYSICAL CHEMISTRY B 113.21(2009):7637-7641. |
入库方式： OAI收割
来源：化学研究所
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