Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column
文献类型：期刊论文
| 作者 | Liu Hai-Yan1; Yang Geng-Liang1,2; Bai Li-Gai1; Feng Xiao-Juan1; Yang Guan-Qun1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 37期号:3页码:325-329 |
| 关键词 | Monolithic Column Separation Immunoglobulin g Immunoglobulin y |
| ISSN号 | 0253-3820 |
| 英文摘要 | Using vinyl ester resin as monomer, ethylene gylcol dimethacrylate (EDMA) as the crosslinker, dodecanol as porogenic solvents and 2, 2' - azobisisobutyronitrile (AIBN) as initiators, a poly (vinyl ester resin-co-ethylene dimethacrylate) monolithic column was prepared by "in-situ" process. The monolith was further derivatized by 3-glycidoxypropyltrimethoxysilane and then hydrolyzed in 0.25 mol/L sulfuric acid to prepare diol column. The effects of pH and buffer concentration on the retention of IgG were investigated and the separation of IgG from human plasma was realized. the separation of IgG from chicken egg yolk in different buffer concentration and the effect of spacer arm on the separation were also investigated. The results showed that the monolith with spacer arm had a higher separation capacity. Moreover, the adsorption capacity of the column was examined and the maximum adsorption amount was 35 mu g/g. |
| 语种 | 英语 |
| WOS记录号 | WOS:000265886400003 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66095]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang Geng-Liang |
| 作者单位 | 1.Hebei Univ, Coll Pharm, Hebei Prov Key Lab Pharmaceut Qual Control, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Hai-Yan,Yang Geng-Liang,Bai Li-Gai,et al. Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(3):325-329. |
| APA | Liu Hai-Yan,Yang Geng-Liang,Bai Li-Gai,Feng Xiao-Juan,&Yang Guan-Qun.(2009).Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(3),325-329. |
| MLA | Liu Hai-Yan,et al."Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.3(2009):325-329. |
入库方式： OAI收割
来源：化学研究所
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