Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes
文献类型：期刊论文
| 作者 | An, Lingling; Liu, Libing; Wang, Shu |
| 刊名 | BIOMACROMOLECULES
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 10期号:2页码:454-457 |
| ISSN号 | 1525-7797 |
| DOI | 10.1021/bm801036h |
| 英文摘要 | A label-free, homogeneous, and sensitive fluorescence "turn-on" approach is designed to rapidly detect protease using serine functionalized polythiophene (POWT). The fluorescence of POWT solution containing bovine serum albumin (BSA) as substrate is efficiently quenched by Cu(2+) ions through coordinate interaction with serine moieties. Upon adding trypsin to the solution, the BSA is cleaved into amino acid or peptide fragments, which are stronger Cu(2+) chelators to form more stable complexes with Cu(2+) ions. Thus, the Cu(2+) ion is displaced from POWT and the fluorescence of POWT is recovered. By triggering the "turn-on" signal of POWT, it is possible to detect the trypsin in real time. "Turn-on" response as readout signal is able to effectively reduce background noise and increase detection sensitivity. Because of the simplicity, high sensitivity, and rapid response, our new enzyme assay shows great potential for protease detection in the future. |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000263226300033 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66119]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Lingling,Liu, Libing,Wang, Shu. Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes[J]. BIOMACROMOLECULES,2009,10(2):454-457. |
| APA | An, Lingling,Liu, Libing,&Wang, Shu.(2009).Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes.BIOMACROMOLECULES,10(2),454-457. |
| MLA | An, Lingling,et al."Label-Free, Homogeneous, and Fluorescence "Turn-On" Detection of Protease Using Conjugated Polyelectrolytes".BIOMACROMOLECULES 10.2(2009):454-457. |
入库方式： OAI收割
来源：化学研究所
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