NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin
文献类型：期刊论文
| 作者 | Lu, Run-Chao1; Guo, Xian-Rong1,2; Jin, Changwen1,2; Xiao, Jin-Xin1,3 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 1790期号:2页码:134-140 |
| 关键词 | Ubiquitin Sodium Perfluorooctanoate Dodecyltrimethylammonium Chloride Unfolding Binding Sites |
| ISSN号 | 0304-4165 |
| DOI | 10.1016/j.bbagen.2008.10.009 |
| 英文摘要 | The fluorinated surfactant sodium perfluorooctanoate (SPFO) Could bind onto ubiquitin (UBQ) and induce the unfolding of UBQ By using (15)N-edited heteronuclear single-quantum coherence (HSQC) NMR and (19)F NMR to monitor (15)N-labeled UBQ and SPFO, respectively, the binding sites and the aggregation process of SPFO on UBQ at various SPFO concentrations were observed. A detailed process from specific binding to cooperative binding of SPFO oil UBQ, and a detailed structure change of UBQ upon the increase of SPFO concentration were obtained. The refolding of UBQ in UBQ-SPFO complex was carried out by adding cationic surfactant. It was shown that added cationic surfactants formed mixed micelles with SPFO and resulted in the dissociation of the UBQ-SPFO complex, and consequently, most ubiquitin could be refolded to its native state. (C) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000262744100007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66161]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xiao, Jin-Xin |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China 3.Beijing FLUOBON Surfactant Inst, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Run-Chao,Guo, Xian-Rong,Jin, Changwen,et al. NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2009,1790(2):134-140. |
| APA | Lu, Run-Chao,Guo, Xian-Rong,Jin, Changwen,&Xiao, Jin-Xin.(2009).NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1790(2),134-140. |
| MLA | Lu, Run-Chao,et al."NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1790.2(2009):134-140. |
入库方式： OAI收割
来源：化学研究所
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