Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy
文献类型：期刊论文
| 作者 | Cao, Rui-Guo; Zhu, Bin; Li, Jingjian; Xu, Dongsheng |
| 刊名 | ELECTROCHEMISTRY COMMUNICATIONS
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 11期号:9页码:1815-1818 |
| 关键词 | Lable-free Dna Biosensor Mercury Electrochemical Impedance Spectroscopy |
| ISSN号 | 1388-2481 |
| DOI | 10.1016/j.elecom.2009.07.029 |
| 英文摘要 | A novel electrochemical biosensor with high sensitivity and selectivity for mercuric ion detection, based on DNA self-assembly electrode, is designed. Thiol functionalized poly-T oligonucleotides were used as gold electrode modifier through formation of Au-S bond between DNA and gold electrode. In presence of Hg(2+) ions, the specific coordination between Hg(2+) and thymine bases can change parallel ss-DNA from linear to hairpin structures, which can cause the release of partial DNA molecules from the surface of the electrode. The density of DNA on the surface of electrode correlated with the concentration of mercury in the solution and can be monitored by electrochemical impedance spectroscopy. The limit of detection of this method is pM level of mercuric ions which is far below the upper limit of Hg(2+) mandated by United States Environmental Protection Agency (EPA), 2 ppb (10 nM). In addition, this method showed excellent selectivity. A series of divalent metal ions, including Ni(2+), Co(2+), Mg(2+), Zn(2+), Ba(2+) and Cd(2+), have little interference with the detection of Hg(2+). (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000270347500023 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66313]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Jingjian |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,et al. Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy[J]. ELECTROCHEMISTRY COMMUNICATIONS,2009,11(9):1815-1818. |
| APA | Cao, Rui-Guo,Zhu, Bin,Li, Jingjian,&Xu, Dongsheng.(2009).Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy.ELECTROCHEMISTRY COMMUNICATIONS,11(9),1815-1818. |
| MLA | Cao, Rui-Guo,et al."Oligonucleotides-based biosensors with high sensitivity and selectivity for mercury using electrochemical impedance spectroscopy".ELECTROCHEMISTRY COMMUNICATIONS 11.9(2009):1815-1818. |
入库方式： OAI收割
来源：化学研究所
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