PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase
文献类型：期刊论文
| 作者 | Lu TianCheng1,2,3; Sun Jing1,3; Dong XiaoQing1,3; Chen XueSi1; Wang Yu1; Jing XiaBin1 |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 52期号:12页码:2033-2037 |
| 关键词 | Electrospun Fibers Glutathione Glutathione S-transferase Protein Separation And Purification |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0291-9 |
| 英文摘要 | The copolymer poly(L-lactic acid)-b-poly(L-cysteine) (PLA-b-PCys) was co-electrospun with PLGA into ultrafine fibers. The reduced glutathione (GSH) was conjugated to the fiber surfaces via disulfide bonds. The glutathione S-transferase (GST) was captured onto the GSH fibers via specific substrate-enzyme interaction between the bound GSH and GST. The captured GST was eluted with free GSH aqueous solution and lyophilized to get pure GST powders. The results show that the GSH moieties on the fiber surface retain the bioactivity of the free GSH and thus they can bind specifically with GST and the GST in solution is captured onto the fiber surface. In addition, the bound GSH is not as active as free GSH so that the captured GST can be eluted off from the fiber by free GSH aqueous solution. Based on this principle, GST itself or its fused proteins can be separated and purified very easily. The preliminary purification efficiency is 6.5 mg center dot(g(PCys))(-1). Further improvements are undertaken. |
| 语种 | 英语 |
| WOS记录号 | WOS:000272917200001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66335]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jing XiaBin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Jilin Agr Univ, Inst Life Sci, Changchun 130118, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu TianCheng,Sun Jing,Dong XiaoQing,et al. PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(12):2033-2037. |
| APA | Lu TianCheng,Sun Jing,Dong XiaoQing,Chen XueSi,Wang Yu,&Jing XiaBin.(2009).PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(12),2033-2037. |
| MLA | Lu TianCheng,et al."PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.12(2009):2033-2037. |
入库方式： OAI收割
来源：化学研究所
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