Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation
文献类型：期刊论文
| 作者 | Qi, Cai1,3,4; Lin, Yi2,5; Feng, Jing3; Wang, Zhan-Hui1; Zhu, Chuan-Feng2; Meng, Yong-Hong1; Yan, Xi-Yun3; Wan, Li-Jun2; Jin, Gang1,3 |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 140期号:1-2页码:79-84 |
| 关键词 | Phage Detection Imaging Ellipsometry Microarray Atomic Force Microscope Microfluidic Surface Modification |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2008.11.010 |
| 英文摘要 | A rapid detection and identification of pathogens is important for minimizing transfer and spread of disease. A label-free and multiplex biosensor based on imaging ellipsometry (BIE) had been developed for the detection of phage M13KO7. The surface of silicon wafer is modified with aldehyde, and proteins can be patterned homogeneously and simultaneously on the surface of silicon wafer in an array format by a microfluidic system. Avidin is immobilized on the surface for biotin-anti-M13 immobilization by means of interaction between avidin and biotin, which will serve as ligand against phage M13KO7. Phages M13KO7 are specifically captured by the ligand when phage M13KO7 solution passes over the surface, resulting in a significant increase of mass surface concentration of the anti-M13 binding phage M13KO7 layer, which could be detected by imaging ellipsometry with a sensitivity of 10(9) pfu/ml. Moreover, atomic force microscopy is also used to confirm the fact that phage M13KO7 has been directly captured by ligands on the surface. It indicates that BIE is competent for direct detection of phage M13KO7 and has potential in the field of virus detection. (C) 2008 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000264650600011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66365]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jin, Gang |
| 作者单位 | 1.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Cai,Lin, Yi,Feng, Jing,et al. Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation[J]. VIRUS RESEARCH,2009,140(1-2):79-84. |
| APA | Qi, Cai.,Lin, Yi.,Feng, Jing.,Wang, Zhan-Hui.,Zhu, Chuan-Feng.,...&Jin, Gang.(2009).Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation.VIRUS RESEARCH,140(1-2),79-84. |
| MLA | Qi, Cai,et al."Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation".VIRUS RESEARCH 140.1-2(2009):79-84. |
入库方式： OAI收割
来源：化学研究所
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