Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template
文献类型：期刊论文
| 作者 | Hua, Zhendong; Zhou, Shuang; Zhao, Meiping |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2009-11-15 |
| 卷号 | 25期号:3页码:615-622 |
| 关键词 | Surface Imprinting Hydrogel Bovine Serum Albumin Silica Microsphere |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2009.01.027 |
| 英文摘要 | Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat Of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K(a)) of 2.2 x 10(5) Lmol(-1) and a maximum binding capacity (Q(max)) of 27.3 mg g(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3 h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials. (C) 2009 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000271931900014 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66371]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hua, Zhendong,Zhou, Shuang,Zhao, Meiping. Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template[J]. BIOSENSORS & BIOELECTRONICS,2009,25(3):615-622. |
| APA | Hua, Zhendong,Zhou, Shuang,&Zhao, Meiping.(2009).Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template.BIOSENSORS & BIOELECTRONICS,25(3),615-622. |
| MLA | Hua, Zhendong,et al."Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template".BIOSENSORS & BIOELECTRONICS 25.3(2009):615-622. |
入库方式： OAI收割
来源：化学研究所
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