Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces
文献类型:期刊论文
作者 | Jing, Benxin1,2; Yang, Qingbo1; Zhu, Yingxi3; Zhao, Jiang1 |
刊名 | JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS
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出版日期 | 2009-12-15 |
卷号 | 47期号:24页码:2541-2546 |
关键词 | Fluorescence Interfaces Polyelectrolytes |
ISSN号 | 0887-6266 |
DOI | 10.1002/polb.21880 |
英文摘要 | The motion of DNA chain under electric field when it is in transient contact with the solid surfaces in aqueous Solution was studied by single molecule fluorescence microscopy at the total internal reflection geometry (TIRFM). In situ observation discovered that single lambda-DNA chains driven by electric field made transient contact with the solid sur-face and made hitting-sliding-leaving-like motion along the surface. By varying the surface chemistry from the negative-charged silanol group-rich surface to positive-charged amino group-rich surface, as well as hydrophobic surfaces, the dependence of DNA mobility on the surface-DNA inter-action was studied. The results show that a dependence of the mobility of DNA oil the surface polarity with respect to DNA itself. The study on different surfaces rich of silanol, amide, amino, and methyl groups show a sequence of DNA mobility of silanol > amide > amino. The mobility of DNA on methyl terminated surface was found to be similar to that on amino surface. (C) 2009 Wiley Periodicals, Inc J Polym Sci Part B Polym Phys 47 2541-2546, 2009 |
语种 | 英语 |
WOS记录号 | WOS:000272561100013 |
出版者 | JOHN WILEY & SONS INC |
源URL | [http://ir.iccas.ac.cn/handle/121111/66387] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Zhao, Jiang |
作者单位 | 1.Chinese Acad Sci, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing Natl Lab Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA |
推荐引用方式 GB/T 7714 | Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,et al. Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces[J]. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,2009,47(24):2541-2546. |
APA | Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,&Zhao, Jiang.(2009).Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces.JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,47(24),2541-2546. |
MLA | Jing, Benxin,et al."Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces".JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS 47.24(2009):2541-2546. |
入库方式: OAI收割
来源:化学研究所
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