Chiral Separation of Calcium (-)-2(S)-2-Benzyl-4-oxo-4(cis-hexahydro-2-isoindolinyl)butyrate Enantiomers by High-performance Liquid Chromatography
文献类型：期刊论文
| 作者 | Zhang Zhefang1; Yang Gengliang1,2; Gao Wenhui3; Chen Yi1 |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 27期号:1页码:29-32 |
| 关键词 | Chiral Separation Coloumn Liquid Chromatography Calcium (-)-2(s)-2-benzyl-4-oxo-4-(Cis-hexahydro-2-isoindolinyl)Butyrate Chiral Stationary Phase |
| ISSN号 | 1001-604X |
| 英文摘要 | A chiral high-performance liquid chromatographic method was developed for the enantioseparation of a new insulinotropic drug of the glinide class with rapid onset. The chiral separation was performed on a Sumichiral OA-3300 column (250 mm x 4.6 mm, 5 mu m) with methanol containing 0.05 mol/L ammonium acetate as the optimized mobile phase at detection wavelength 210 nm. Baseline separation of the two enantiomers was obtained in 22 min with a resolution of 3.01. Calibration graphs were constructed in a range of 0.028-5.6 mu g center dot mL(-1) for S- and 0.03-6.0 mu g center dot mL(-1) for R-(-)-enantiomer, respectively. The linear correlation equations are: y=1.32x10(3)x(-)2.54 r=0.9997) for S-enantiomer and y=1.15x10(3)x(-)1.78 (r=0.9998) for R-enantiomer, respectively. The limits of detection obtained by S/N=3 were 0.15 no for S- and 0.10 no for R-enantiomer, respectively. RSD of the method was below 1.0% (n = 5). |
| 语种 | 英语 |
| WOS记录号 | WOS:000264039400006 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66437]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang Gengliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100190, Peoples R China 2.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Hebei, Peoples R China 3.Hebei Univ Sci & Technol, Coll Biol Sci & Engn, Shijiazhuang 050018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Zhefang,Yang Gengliang,Gao Wenhui,et al. Chiral Separation of Calcium (-)-2(S)-2-Benzyl-4-oxo-4(cis-hexahydro-2-isoindolinyl)butyrate Enantiomers by High-performance Liquid Chromatography[J]. CHINESE JOURNAL OF CHEMISTRY,2009,27(1):29-32. |
| APA | Zhang Zhefang,Yang Gengliang,Gao Wenhui,&Chen Yi.(2009).Chiral Separation of Calcium (-)-2(S)-2-Benzyl-4-oxo-4(cis-hexahydro-2-isoindolinyl)butyrate Enantiomers by High-performance Liquid Chromatography.CHINESE JOURNAL OF CHEMISTRY,27(1),29-32. |
| MLA | Zhang Zhefang,et al."Chiral Separation of Calcium (-)-2(S)-2-Benzyl-4-oxo-4(cis-hexahydro-2-isoindolinyl)butyrate Enantiomers by High-performance Liquid Chromatography".CHINESE JOURNAL OF CHEMISTRY 27.1(2009):29-32. |
入库方式： OAI收割
来源：化学研究所
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