Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB Receptors HER3/HER2 by single-molecule force spectroscopy
文献类型：期刊论文
| 作者 | Shi, Xiaoli; Xu, Li; Yu, Junping; Fang, Xiaohong |
| 刊名 | EXPERIMENTAL CELL RESEARCH
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 315期号:16页码:2847-2855 |
| 关键词 | Epidermal Growth Factor Receptor Heregulin Herceptin Atomic Force Microscopy Single-molecule Force Spectroscopy Ligand-receptor Interaction |
| ISSN号 | 0014-4827 |
| DOI | 10.1016/j.yexcr.2009.05.023 |
| 英文摘要 | Herceptin is a monoclonal antibody against HER2, which is a member of the epidermal growth factor receptor (ErbB) family and is overexpressed in many cancers. In this work, we have applied single molecule force spectroscopy 10 study the effect of Herceptin oil HER2 modulated ligand-receptor interaction for ErbB signaling in living cells. Heregulin beta 1 (HRG), the specific ligand of HER3, was used for HER2 activation as HER3 is the preferable dimerization partner of HER2 and HER3/HER2 is the most representative heterodimer found in cancer. Our results demonstrated a more stable binding of HRG to the cells co-expressing HER3 and HER2 than those expressing HER3 alone. Moreover, the binding force of Herceptin and HER2 is as strong as that of HRG and HER3/HER2. With the addition of Herceptin, the binding strength of HRG to the cells co-expressing HER3 and HER2 decreased. The presence of Herceptin changed the dynamic force spectrum of HRG-HER3/HER2 to that similar to HRG-HER3. Therefore, the enhancement in HRG-HER3 binding after recruiting HER2 was inhibited by Herceptin. The method offers a new approach to Study the molecular mechanism of Herceptin anti-cancer effect. (C) 2009 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000275553900015 |
| 出版者 | ELSEVIER INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66669]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiaoli,Xu, Li,Yu, Junping,et al. Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB Receptors HER3/HER2 by single-molecule force spectroscopy[J]. EXPERIMENTAL CELL RESEARCH,2009,315(16):2847-2855. |
| APA | Shi, Xiaoli,Xu, Li,Yu, Junping,&Fang, Xiaohong.(2009).Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB Receptors HER3/HER2 by single-molecule force spectroscopy.EXPERIMENTAL CELL RESEARCH,315(16),2847-2855. |
| MLA | Shi, Xiaoli,et al."Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB Receptors HER3/HER2 by single-molecule force spectroscopy".EXPERIMENTAL CELL RESEARCH 315.16(2009):2847-2855. |
入库方式： OAI收割
来源：化学研究所
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