Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei
文献类型：期刊论文
| 作者 | Wang, Yanli1; Wang, Jia1; Deng, Xiaoyong1; Wang, Jiao2; Wang, Haifang1; Wu, Minghong1; Jiao, Zheng1; Liu, Yuanfang1,3 |
| 刊名 | NANO RESEARCH
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 2期号:7页码:543-552 |
| 关键词 | Titania Nanotubes Mouse Neural Stem Cells Nucleus Confocal Imaging Atomic Force Microscopy |
| ISSN号 | 1998-0124 |
| DOI | 10.1007/s12274-009-9052-5 |
| 英文摘要 | Titania nanotubes (TiO2-NTs) are a potential drug vehicle for use in nanomedicine. To this end, a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out. TiO2-NTs were first conjugated with a fluorescent label, fluorescein isothiocyanate (FITC). FITC-conjugated titania nanotubes (FITC-TiO2-NTs) internalized in mouse neural stem cells (NSCs, line C17.2) can be directly imaged by confocal microscopy. The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells. After co-incubation with cells for 24 h, FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca. More interestingly, the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) were also employed in tracking the nanotubes in the cell. These results will be of benefit in future studies of TiO2-NTs for use as a drug vehicle, particularly for DNA-targeting drugs. |
| 语种 | 英语 |
| WOS记录号 | WOS:000273939500004 |
| 出版者 | TSINGHUA UNIV PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66869]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Yuanfang |
| 作者单位 | 1.Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China 2.Shanghai Univ, Inst Syst Biol, Shanghai 200444, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yanli,Wang, Jia,Deng, Xiaoyong,et al. Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei[J]. NANO RESEARCH,2009,2(7):543-552. |
| APA | Wang, Yanli.,Wang, Jia.,Deng, Xiaoyong.,Wang, Jiao.,Wang, Haifang.,...&Liu, Yuanfang.(2009).Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei.NANO RESEARCH,2(7),543-552. |
| MLA | Wang, Yanli,et al."Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei".NANO RESEARCH 2.7(2009):543-552. |
入库方式： OAI收割
来源：化学研究所
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