Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures
文献类型：期刊论文
| 作者 | Ren, Yanan1; Zhang, Chunxi1; Bao, Han1; Shen, Jianren2; Zhao, Jingquan1 |
| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 99期号:2页码:127-138 |
| ISSN号 | 0166-8595 |
| 关键词 | Photosystem Ii Core Complex Tyrosine z Spinach Epr |
| DOI | 10.1007/s11120-009-9410-x |
| 英文摘要 | Tyrosine Z (Tyr(Z)) oxidation observed at liquid helium temperatures provides new insights into the structure and function of Tyr(Z) in active Photosystem II (PSII). However, it has not been reported in PSII core complex from higher plants. Here, we report Tyr(Z) oxidation in the S(1) and S(2) states in PSII core complex from spinach for the first time. Moreover, we identified a 500 G-wide symmetric EPR signal (peak position g = 2.18, trough position g = 1.85) together with the g = 2.03 signal induced by visible light at 10 K in the S(1) state in the PSII core complex. These two signals decay with a similar rate in the dark and both disappear in the presence of 6% methanol. We tentatively assign this new feature to the hyperfine structure of the S(1)Tyr(Z) (aEuro cent) EPR signal. Furthermore, EPR signals of the S(2) state of the Mn-cluster, the oxidation of the non-heme iron, and the S(1)Tyr(Z) (aEuro cent) in PSII core complexes and PSII-enriched membranes from spinach are compared, which clearly indicate that both the donor and acceptor sides of the reaction center are undisturbed after the removal of LHCII. These results suggest that the new spinach PSII core complex is suitable for the electron transfer study of PSII at cryogenic temperatures. |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000263544900006 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/66905]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Chunxi |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Photochem, Beijing 100190, Peoples R China 2.Okayama Univ, Grad Sch Nat Sci & Technol, Fac Sci, Dept Biol, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Ren, Yanan,Zhang, Chunxi,Bao, Han,et al. Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures[J]. PHOTOSYNTHESIS RESEARCH,2009,99(2):127-138. |
| APA | Ren, Yanan,Zhang, Chunxi,Bao, Han,Shen, Jianren,&Zhao, Jingquan.(2009).Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures.PHOTOSYNTHESIS RESEARCH,99(2),127-138. |
| MLA | Ren, Yanan,et al."Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures".PHOTOSYNTHESIS RESEARCH 99.2(2009):127-138. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。