Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration
文献类型：期刊论文
| 作者 | Wang, Qinli1; Chen, Bo2; Liu, Peng1; Zheng, Maozhong1; Wang, Yuqing1; Cui, Sujuan3; Sun, Daye3; Fang, Xiaohong2; Liu, Chun-Ming1; Lucas, William J.4 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 284期号:18页码:12000-12007 |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.M808028200 |
| 英文摘要 | Calmodulin (CaM) is a highly conserved intracellular calcium sensor. In plants, CaM also appears to be present in the apoplasm, and application of exogenous CaM has been shown to influence a number of physiological functions as a polypeptide signal; however, the existence and localization of its corresponding apoplasmic binding sites remain controversial. To identify the site(s) of action, a CaM-conjugated quantum dot (QD) system was employed for single molecule level detection at the surface of plant cells. Using this approach, we show that QD-CaM binds selectively to sites on the outer surface of the plasma membrane, which was further confirmed by high resolution transmission electron microscopy. Measurements of Ca(2+) fluxes across the plasma membrane, using ion-selective microelectrodes, demonstrated that exogenous CaM induces a net influx into protoplasts. Consistent with these flux studies, calcium-green-dextran and FRET experiments confirmed that applied CaM/QD-CaM elicited an increase in cytoplasmic Ca(2+) levels. These results support the hypothesis that apoplasmic CaM can act as a signaling agent. These findings are discussed in terms of CaM acting as an apoplasmic peptide ligand to mediate transmembrane signaling in the plant kingdom. |
| 语种 | 英语 |
| WOS记录号 | WOS:000265494600032 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67031]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lin, Jinxing |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Mol Environm Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.Hebei Normal Univ, Inst Mol Cell Biol, Shijiazhuang 050016, Hebei Province, Peoples R China 4.Univ Calif Davis, Dept Plant Biol, Coll Biol Sci, Davis, CA 95616 USA |
| 推荐引用方式 GB/T 7714 | Wang, Qinli,Chen, Bo,Liu, Peng,et al. Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(18):12000-12007. |
| APA | Wang, Qinli.,Chen, Bo.,Liu, Peng.,Zheng, Maozhong.,Wang, Yuqing.,...&Lin, Jinxing.(2009).Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration.JOURNAL OF BIOLOGICAL CHEMISTRY,284(18),12000-12007. |
| MLA | Wang, Qinli,et al."Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration".JOURNAL OF BIOLOGICAL CHEMISTRY 284.18(2009):12000-12007. |
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来源：化学研究所
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