Strategy for Molecular Beacon Binding Readout: Separating Molecular Recognition Element and Signal Reporter
文献类型：期刊论文
| 作者 | Wang, Yongxiang1; Li, Jishan2; Jin, Jianyu1; Wang, Hao1; Tang, Hongxing2; Yang, Ronghua1,2; Wang, Kemin2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 81期号:23页码:9703-9709 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac901906w |
| 英文摘要 | A new strategy for molecular beacon binding readout is proposed by using separation of the molecular recognition element and signal reporter. The signal transduction of the target binding event is based on displacing interaction between the target DNA and a competitor, the signal transducer. The target-free capture DNA is first interacted with the competitor, forming an assembled complex. In the presence of a target DNA that the affinity is stronger than that of the competitor, hybridization between capture DNA and the target disassembles the assembled complex and releases the free competitor to change the readout of the signal reporter. To demonstrate the feasibility of the design, a thymine-rich oligonucleotide was examined as a model system. Hg(2+) was selected as the competitor, and mercaptoacetic acid-coated CdTc/ZnS quantum dots served as the fluorescent reporter. Selective binding of Hg(2+) between the two thymine bases of the capture DNA forms a hairpin-structure. Hybridization between the capture DNA and target DNA destroys the hairpin-structure, releasing Hg(2+) ions to quench the quantum dots fluorescence. Under the optimal conditions, fluorescence intensity of the quantum dots against the concentration of perfect cDNA was linear over the concentration range of 0.1-1.6 mu M, with a limit of detection of 25 nM. This new assay method is simple in design, avoiding any oligonucleotide labeling. Furthermore, this strategy is generalizable since any target binding can in principle release the signal transducer and be detected with separated signal reporter. |
| 语种 | 英语 |
| WOS记录号 | WOS:000272084700029 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67039]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Ronghua |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yongxiang,Li, Jishan,Jin, Jianyu,et al. Strategy for Molecular Beacon Binding Readout: Separating Molecular Recognition Element and Signal Reporter[J]. ANALYTICAL CHEMISTRY,2009,81(23):9703-9709. |
| APA | Wang, Yongxiang.,Li, Jishan.,Jin, Jianyu.,Wang, Hao.,Tang, Hongxing.,...&Wang, Kemin.(2009).Strategy for Molecular Beacon Binding Readout: Separating Molecular Recognition Element and Signal Reporter.ANALYTICAL CHEMISTRY,81(23),9703-9709. |
| MLA | Wang, Yongxiang,et al."Strategy for Molecular Beacon Binding Readout: Separating Molecular Recognition Element and Signal Reporter".ANALYTICAL CHEMISTRY 81.23(2009):9703-9709. |
入库方式： OAI收割
来源：化学研究所
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