Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c
文献类型：期刊论文
| 作者 | Qin ChuanJiang; Tong Hui; Wang LiXiang |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 52期号:6页码:833-839 |
| 关键词 | Conjugated Polymer Fluorescence Sensor Water-soluble Side Chain |
| ISSN号 | 1006-9291 |
| DOI | 10.1007/s11426-009-0067-2 |
| 英文摘要 | An anionic water-soluble polyfluorene derivative, poly(9,9-bis(6'-phosphatehexyl)fluorene-alt-1,4-phenylene) sodium salt (PFHPNa), was synthesized by Suzuki coupling reaction in DMF/water. Polymer PFHPNa was well soluble in water with a strong blue fluorescence emission. Effect of the side chain length on fluorescence sensory properties was studied by comparing quenching efficiencies toward different quenchers of PFHPNa with a reported polymer poly(9,9-bis(3'-phosphatepropyl)fluorene-alt-1,4-phenylene) sodium salt (PFPPNa), which have different side chains in length. For small molecular quenchers (methylviologen, MV(2+)) and meso-5,10,15,20-tetrakis-(N-methyl-4-pyridyl)porphine (TMPyP4), polymer PFHPNa had lower sensitivity due to the much longer side chain length. The positively charged metalloprotein cytochrome c could quench fluorescence of conjugated polymers via energy transfer and electron transfer. Moreover, polymer PFHPNa showed higher fluorescence quenching toward large biomolecules than PFPPNa. The corresponding Stern-Volmer (K (sv)) value of polymer PFHPNa was determined to be 2.1x10(8) M(-1) for cytochrome c. It could be used as a sensitive and selective fluorescence sensor for protein cytochrome c. |
| 语种 | 英语 |
| WOS记录号 | WOS:000266168000021 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67067]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang LiXiang |
| 作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin ChuanJiang,Tong Hui,Wang LiXiang. Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):833-839. |
| APA | Qin ChuanJiang,Tong Hui,&Wang LiXiang.(2009).Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),833-839. |
| MLA | Qin ChuanJiang,et al."Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):833-839. |
入库方式： OAI收割
来源：化学研究所
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