中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex

文献类型:期刊论文

作者Qi, Li1,2; Yang, Gengliang1
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2009-09-01
卷号32期号:18页码:3209-3214
关键词Dansyl Amino Acid Enantioseparation Ligand-exchange Capillary Electrophoresis Zinc-l-phenylalaninamide Complex
ISSN号1615-9306
DOI10.1002/jssc.200900328
英文摘要A novel method of chiral ligand-exchange CE was developed with L-amino acylamides as a chiral ligand and zinc(II) as a central ion. It has been demonstrated that these chiral complexes, such as Zn(II)-L-alaninamide, Zn(II)-L-prolinamide, and Zn(II)-L-phenylalaninamide, are suitable for use as chiral selectors for the enantioseparation of either individual pair of or mixed dansyl amino acids. The optimal separation running buffer consisted of 5 mM ammonium acetate, 100 mM boric acid, 4 mM ZnSO(4) center dot 7H(2)O, and 8 mM L-amino acylamides at pH 8.2. The experiments showed that apart from the effect of the concentration of the complexes on the resolution and the migration time, the buffer pH also had a sharp influence on resolution. The employed chiral ligands exhibited different enantioselectivities and enantiomer migration orders. D-Amino acids migrate faster than L-amino acids when Zn(II)-L-alaninamide and Zn(II)-L-phenylalaninamide are used as chiral selectors, but it was observed that the migration order is reversed when Zn(II)-L-prolinamide is used as the chiral selector. Furthermore, the amount of dansylated amino acids is found to be highly dependent on the labeling temperature.
语种英语
WOS记录号WOS:000270375300018
出版者WILEY-V C H VERLAG GMBH
源URL[http://ir.iccas.ac.cn/handle/121111/67075]  
专题中国科学院化学研究所
通讯作者Yang, Gengliang
作者单位1.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Qi, Li,Yang, Gengliang. Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(18):3209-3214.
APA Qi, Li,&Yang, Gengliang.(2009).Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex.JOURNAL OF SEPARATION SCIENCE,32(18),3209-3214.
MLA Qi, Li,et al."Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex".JOURNAL OF SEPARATION SCIENCE 32.18(2009):3209-3214.

入库方式: OAI收割

来源:化学研究所

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