A Novel Method for Enzyme Design
文献类型：期刊论文
| 作者 | Zhu, Xiaolei1,2; Lai, Luhua1,2 |
| 刊名 | JOURNAL OF COMPUTATIONAL CHEMISTRY
 |
| 出版日期 | 2009-01-30 |
| 卷号 | 30期号:2页码:256-267 |
| 关键词 | Enzyme Design Vector Matching Active Site Residues Coslscore Triose Phosphate Isomerase Protein Scaffold Side-chain Packing |
| ISSN号 | 0192-8651 |
| DOI | 10.1002/jcc.21050 |
| 英文摘要 | Rational design of enzymes is a stringent test of our understanding of protein structure and function relationship, which also has numerous potential applications. We present a novel method for enzyme design that call find good candidate protein scaffolds in a protein-ligand database based on vector matching of key residues. Residues in the vicinity of the active site were also compared according to a similarity score between the scaffold protein and the target enzyme. Suitable scaffold proteins were selected, and the side chains of residues around the active sites were rebuilt using a previously developed side-chain packing program. Triose phosphate isomerase (TIM) was used as a validation test for enzyme design. Selected scaffold proteins were found to accommodate the enzyme active sites and successfully form a good transition state complex. This method overcomes the limitations of the Current enzyme design methods that use limited number of protein scaffold and based on the position of ligands. As there are a large number of protein scaffolds available in the Protein Data Band, this method should be widely applicable for various types of enzyme design. (C) 2008 Wiley Periodicals, Inc. J Comput Chem 30: 256-267, 2009 |
| 语种 | 英语 |
| WOS记录号 | WOS:000262198400009 |
| 出版者 | JOHN WILEY & SONS INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67227]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lai, Luhua |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China 2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xiaolei,Lai, Luhua. A Novel Method for Enzyme Design[J]. JOURNAL OF COMPUTATIONAL CHEMISTRY,2009,30(2):256-267. |
| APA | Zhu, Xiaolei,&Lai, Luhua.(2009).A Novel Method for Enzyme Design.JOURNAL OF COMPUTATIONAL CHEMISTRY,30(2),256-267. |
| MLA | Zhu, Xiaolei,et al."A Novel Method for Enzyme Design".JOURNAL OF COMPUTATIONAL CHEMISTRY 30.2(2009):256-267. |
入库方式： OAI收割
来源：化学研究所
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