Arene Control over Thiolate to Sulfinate Oxidation in Albumin by Organometallic Ruthenium Anticancer Complexes
文献类型：期刊论文
| 作者 | Hu, Wenbing1; Luo, Qun1; Ma, Xiaoyan1; Wu, Kui1; Liu, Jianan1; Chen, Yi1; Xiong, Shaoxiang1; Wang, Jianping1; Sadler, Peter J.2; Wang, Fuyi1 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2009 |
| 卷号 | 15期号:27页码:6586-6594 |
| 关键词 | Albumin Antitumor Agents Bioinorganic Chemistry Mass Spectrometry Ruthenium |
| ISSN号 | 0947-6539 |
| DOI | 10.1002/chem.200900699 |
| 英文摘要 | Interactions of organometallic ruthenium anticancer complexes [Ru(eta(6)-arcne)Cl(en)][PF(6)] (arene = p-cymene (1) or biphenyl (2), en=ethylenediamine) with human serum albumin were investigated by means of mass spectrometry combined with trypsin digestion, specific sidechain modifications and computational modelling. Both complexes were shown to bind to surface histidine (His128, His247, His510) and methionine (Met298) residues in human albumin, but only the p-cymene complex can gain entry to the crevice containing the free cysteine thiolate (Cys34) and induce oxidation to sulfinate. The two complexes exhibit a similar coordination preference for histidine and methionine residues on the protein surface. His128 binding is favoured both kinetically and thermodynamically. At 310 K, six days of incubation of recombinant human albumin (rHA) with complex I (rHA:Ru 50:250 pm) led to about 18% ruthenation of His128 in the protein. However, the extent of ruthenation of albumin by complex 2 was less than that by I., due to the steric hindrance from the biphenyl ligand. These results imply that the arene ligand in the organometallic ruthenium anticancer complexes plays a crucial role in interactions with proteins. |
| 语种 | 英语 |
| WOS记录号 | WOS:000267927500008 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/67431]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Jianping |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China 2.Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Hu, Wenbing,Luo, Qun,Ma, Xiaoyan,et al. Arene Control over Thiolate to Sulfinate Oxidation in Albumin by Organometallic Ruthenium Anticancer Complexes[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(27):6586-6594. |
| APA | Hu, Wenbing.,Luo, Qun.,Ma, Xiaoyan.,Wu, Kui.,Liu, Jianan.,...&Wang, Fuyi.(2009).Arene Control over Thiolate to Sulfinate Oxidation in Albumin by Organometallic Ruthenium Anticancer Complexes.CHEMISTRY-A EUROPEAN JOURNAL,15(27),6586-6594. |
| MLA | Hu, Wenbing,et al."Arene Control over Thiolate to Sulfinate Oxidation in Albumin by Organometallic Ruthenium Anticancer Complexes".CHEMISTRY-A EUROPEAN JOURNAL 15.27(2009):6586-6594. |
入库方式： OAI收割
来源：化学研究所
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